Evaluation of Lo＂wenstein-Jensen Medium Culture,MGIT 960 Culture and Different Specimen Types inDiagnosis of Bone and Joint Tuberculosis
作者机构：National Clinical Laboratory on Tuberculosis Beijing Key laboratory for Drug-resistant Tuberculosis Research Beijing ChestHospital Capital Medical University Beijing Tuberculosis and Thoracic Tumor Institute Beijing 101149 China Department of orthopedics Beijing Bone and Joint Tuberculosis Diagnosis and Treatment Center Beijing Chest Hospital CapitalMedical University Beo'ing Tuberculosis and Thoracic Tumor Institute Beijing 101149 China
出 版 物：《Journal of Pharmacy and Pharmacology》 (药剂与药理学（英文版）)
年 卷 期：2015年第3卷第3期
页 面：128-132页
学科分类：10[医学]
主 题：Bone and joint tuberculosis, mycobacteria, specimen type, culture.
摘 要：Objective： The aim of this study was to evaluate L-J （Lo＂wenstein-Jensen） medium culture, MGIT 960 culture anddifferent specimen types in diagnosis of BJTB （bone and joint tuberculosis）. Methods：： Specimens of pus, caseous necrosis,tuberculous granuloma and sequestrum were collected from 52 BJTB patients. All specimens were cultured using both MGIT 960system and L-J medium; and all pus were amplified using real-time PCR to detect the presence of M. tuberculosis DNA. KeyFindings： A total of 191 specimens were collected. Granuloma had better chance to produce positive outcomes by L-J mediumculture, while for sequestrum MGIT 960 culture had higher yield, but there was no significant difference in the recovery rates amongdifferent types of specimen either by L-J culture （Z2 = 0.638, P = 0.888） or by MGIT960 culture （Z2 = 1.399, P = 0.706）. MGIT960culture had significantly higher recovery rate than L-J culture, With a combined culture and PCR-based test, the recovery rate of pusspecimen was significantly higher than that of either method alone （P 〈 0.05）. Conclusion： MGIT 960 culture is superior to L-Jculture in BJTB diagnosis; pus, sequestrum, granuloma and caseous necrosis are usable specimen for mycobacterial culture;combination of culture and molecular techniques can provide a better diagnostic significance.
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