Effect of 1,25-Dihydroxyvitamin D_3 on Regulatory T Cells in Ovariectomized Mice

作     者：LIU Jun Chen ZHOU Chen Hui ZHANG Xue CHEN Yan XU Bi Lian CUI Liao XU Dao Hua 

作者机构：Department of Pharmacology Guangdong Medical College The Second Affiliated Hospital (Jiande Branch) School of Medicine ZheJiang University Institution of Traditional Chinese Medicine and New Pharmacy Development Guangdong Medical College 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2014年第27卷第10期

页      面：779-785页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by Natural Science Foundation of Guangdong Province(10152402301000000) Science and Technology Planning Project of Dongguan(2011108102019) Science&Technology Innovation Fund of Guangdong Medical College(STIF201104) 

主　　题：1,25-dihydroxyvitamin D31 Osteoporosis Regulatory T cells Ovariectomy 

摘      要：Objective To investigate the correlation between regulatory T （Treg） cells and postmenopausal osteoporosis and the antiosteoporotic effect of 1,25-dihydroxyvitamin D3 [1,25（OH）2D3] in relation to Treg cells. Methods Fifty female BALB/c mice were randomly divided into five groups： the basal control （BAS）, Sham, ovariectomy （OVX）, OVX＋diethylstilbestrol （OVX＋DES）, and OVX＋I,2S（OH）2D3. Tibias were harvested and processed with decalcification for quantitative bone histomorphometry. Femurs were stained by immunohistochemistry to detect Foxp3 protein expression. Spleens were used to detect Treg and Foxp3 gene expression by flow cy：ometry and quantitative RT-PCR, respectively. Results In comparison with the Sham group, a significant decrease was found in the OV~ group in such indices as trabecular bone volume/tc,tal tissue area （BV/TV）, trabecular number （Tb.N） and trabecular thickness （***）. 1,25（OH）2D3 and DES partly prevented the decrease in BV/TV, Tb.N, *** in OVX mice. Treg cell number, Foxp3 mRI~：A expression in spleen and Foxp3 protein expression in femur significantly decreased in the OVX-tr^ated group compared with those in the sham group. 1,25（OH）2D3 and DES significantly increased Treg cell number and Foxp3 expression. Treg cells and Foxp3 gene expression were related to bone histomorphometric parameters. Conclusion The decrease in Treg cell numbers is relevant to the postmenopausal osteoporosis. The antiosteoporosis of 1,25（OH）2D3 is related to regulatory T cells.