Molecular Scrippsiella phylogenetic analysis of dinoflagellate trochoidea isolated from the East Asianwaters

作     者：TANG Xianghai YURencheng ZHANG Qingchun WANG Yunfeng YAN Tian ZHOU Mingjiang 

作者机构：Key Laboratory of Marine Ecology and Environmental Sciences Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Graduate School of Chinese Academy of Sciences Beijing 100039 China 

出 版 物：《Chinese Journal of Oceanology and Limnology》 (中国海洋湖沼学报（英文版）)

年 卷 期：2010年第28卷第2期

页      面：323-328页

核心收录：

学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：Supported by the Knowledge Innovation Program of Chinese Academy of Sciences (No. KZCX2-YW-208) and National Natural Science Foundation of China (No. 40676072) 

主　　题：Serippsiella trochoidea phylogeny rDNA molecular systematics 

摘      要：Previous studies found intraspecific diversity in Scrippsiella trochoidea A. R. Loeblich Ill, a widely distributed calcareous cyst-producing dinoflagellate. In this study, three strains （ST-I, ST-D6 and ST-K） of S. trochoidea isolated from the East Asian waters were studied, together with other geographical strains, to resolve their phylogenetic relationships. For the three East Asian isolates, two highly diverse regions of nuclear-encoded ribosomal DNA （rDNA）, the 5.8S rDNA and its flanking internal transcribed spacers 1 and 2, and the 5＇ portion of the large-subunit rDNA （encompassing the ＂DI＂ and ＂D2＂ domains） were sequenced. Homologous sequences from other geographical isolates were selected from the GenBank database and the phylogenetic relationships were inferred from the molecular data of these strains. Strains of S. trochoidea were found to cluster into three major clades （STR1, STR2 and STR3）, as reported in previous studies. Two of the three strains ST-1 and ST-K, were grouped in clade STR2, the other strain, ST-D6, belonged to clade STR3. The intraspecific diversity ofS. trochoidea in East Asian waters makes it necessary to carry out phylogenetic investigations in combination with data of biogeography, population dynamics, and life cycle on the ecophysiology of a region.