Differences in oxidative stress dependence between gastric adenocarcinoma subtypes

作     者：Brigitte Bancel JacauesEstève Jean-Christophe Souquet Shinya Toyokuni Hiroshi Ohshima Brigitte Pignatelli 

作者机构：Hospital de la Croix-RousseDepartment of PathologyLyonFrance Centre Hospital de la Croix-RousseDepartment of GastroenterologyLyonFrance Department of Pathology and Biology of DiseasesGraduate School of MedicineKyoto UniversityKyotoJapan Brigitte PignatelliInternational Agency for Research on Cancer.Endogenous Risk Factors GroupLyon.France Clinical Epidemiology UnitDepartment of Medical InformationHospices Civils de LyonFrance 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2006年第12卷第7期

页      面：1005-1012页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Gastric neoplasms Nitric oxide synthase Hpylori Nitrotyrosine 8-hydroxydeoxyguanosine Oxidative stress 

摘      要：AIM： To investigate the extent of oxidative stress in preneoplastic and neoplastic gastric mucosa in relation to their pathological criteria and histological subtypes. METHODS： A total of 104 gastric adenocarcinomas from 98 patients （88 infiltrative and 16 intraepithelial tumors） were assessed immunohistochemically for expression of iNOS and occurrence of nitrotyrosine （NTYR）-containing proteins and 8-hydroxy-2＇-deoxyguanosine （8-OH- dG）-containing DNA, as markers of NO production and damages to protein and DNA. RESULTS： Tumor cells staining for iNOS, NTYR and 8-OH-dG were detected in 41%, 62% and 50% of infiltrative carcinoma, respectively. The three markers were shown for the first time in intraepithelial carcinoma. The expression of iNOS was significantly more frequent in tubular carcinoma （TC） compared to diffuse carcinoma （DC） （54% vs 18%; P=0.008） or in polymorphous carcinoma （PolyC） （54% vs 21% ; P=0.04）. NTYR staining was obviously more often found in TC than that in PolyC （72% vs 30% ; P=0.03）. There was a tendency towards a higher rate of iNOS staining when distant metastasis （pM） was present. In infiltrative TC, the presence of oxidative stress markers was not significantly correlated with histological grade, density of inflammation, the depth of infiltration （pT）, lymph nodes dissemination （pN） and pathological stages （pTNM）. CONCLUSION： The iNOS-oxidative pathway may play an important role in TC, but moderately in PolyC and DC. DNA oxidation and protein nitration occur in the three subtypes. Based on the significant differences of NTYR levels, TC and PolyC appear as two distinct subtypes.