Diagnosis and treatment of refractory infectious diseases using nanopore sequencing technology:Three case reports
作者机构：Science Island BranchGraduate School of University of Science and Technology of ChinaHefei 230031Anhui ProvinceChina Institute of Health and Medical TechnologyHefei Institutes of Physical ScienceChinese Academy of ScienceHefei 230031Anhui ProvinceChina Medical Pathology CenterHefei Cancer HospitalChinese Academy of SciencesHefei 230031Anhui ProvinceChina
出 版 物：《World Journal of Clinical Cases》 (世界临床病例杂志)
年 卷 期：2024年第12卷第22期
页 面：5208-5216页
学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 08[工学] 100401[医学-流行病与卫生统计学] 0836[工学-生物工程] 10[医学]
基 金：Supported by Research and Development Funding for Medical and Health Institutions No.2021YL007
主 题：Nanopore sequencing technology Third-generation sequencing technology Infection Pathogen Case report
摘 要：BACKGROUND Infectious diseases are still one of the greatest threats to human health,and the etiology of 20%of cases of clinical fever is unknown;therefore,rapid identification of pathogens is highly *** culture methods are only able to detect a limited number of pathogens and are time-consuming;serologic detection has window periods,false-positive and false-negative problems;and nucleic acid molecular detection methods can detect several known pathogens only ***-generation nanopore sequencing technology provides new options for identifying *** SUMMARY Case 1:The patient was admitted to the hospital with abdominal pain for three days and cessation of defecation for five days,accompanied by cough and *** sequencing of the drainage fluid revealed the presence of orallike bacteria,leading to a clinical diagnosis of bronchopleural *** sodium sulbactam treatment was *** 2:The patient was admitted to the hospital with fever and headache,and CT revealed lung *** treatment for Streptococcus pneumoniae,identified through nanopore sequencing of cerebrospinal fluid,was *** 3:The patient was admitted to our hospital with intermittent fever and an enlarged neck mass that had persisted for more than six *** antibacterial treatment,her symptoms *** nanopore sequencing results indicate that voriconazole treatment is effective for Aspergillus *** patient was diagnosed with mixed cell type classical Hodgkin s lymphoma with *** Three-generation nanopore sequencing technology allows for rapid and accurate detection of pathogens in human infectious diseases.
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