Cloning and characterization of a novel barley gene,HvORG4,induced by Fusarium graminearum infection
Cloning and characterization of a novel barley gene,HvORG4,induced by Fusarium graminearum infection作者机构：Plant Research International B.V.WageningenThe Netherlands
出 版 物：《湖南农业大学学报（自然科学版）》 (Journal of Hunan Agricultural University(Natural Sciences))
年 卷 期：2007年第33卷第S1期
页 面：212-页
主 题：cDNA Cloning and characterization of a novel barley gene,HvORG4,induced by Fusarium graminearum infection gene
摘 要：Barley Fusarium head blight(FHB),caused by species of the Fusarium fungus,is a devastating disease that is reemerging worldwide in recent *** this study,a novel gene,HvORG4,was cloned from barley by using cDNA library and suppression subtractive hybridization(SSH) library *** SSH library and cDNA library were constructed from the Chinese barley cultivar Jing02-461(resistance to FHB) infected by Fusarium graminearum isolate *** the SSH analysis,more than 120 differentially expressed cDNAs were identified and *** of them showed high homology to the AtORG4 gene and was used as a probe to screen the cDNA library of *** positive clones were identified and one of them contained a full-length cDNA,which was named *** analysis showed that HvORG4 encoded a deduced basic protein of 197 amino *** blotting analysis showed that HvORG4 was constitutively expressed in root and stalk,not in leaf or spike,and strongly induced in barley spikelets in response to infection with *** isolate *** homology and expression profile suggest that the HvORG4 might function as a transcription factor,playing an important role in signal transduction pathway for defense against FHB in barley.
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