Evaluation of Rapid Detection of Nasopharyngeal Colonization with MRSA by Real-Time PCR

作     者：Feng-feng Kang Shu-yan Cao Yan-ming Li Jiang-tao Li Qing He Yi Li Yun-jian Hu 

作者机构：Beijing HospitalMinistry of Health Institution of Laboratory MedicineXiangya School of MedicineCentral South University 

出 版 物：《国际感染病学（电子版）》 (Infection International(Electronic Edition))

年 卷 期：2012年第1卷第1期

页      面：-页

学科分类：100208[医学-临床检验诊断学] 1002[医学-临床医学] 10[医学] 

主　　题：Real-Time PCR MRSA Nasopharyngeal swab 

摘      要：Objective To investigate the clinical application of Real-Time PCR for rapid detection of methicillin-resistant Staphylococcus aureus(MRSA) directly from nasopharyngeal swab *** We collected the nasal and throat swab specimens from patients or medical staffs in 3 intensive care units,blood laminar flow ward and respiratory ward in Beijing Hospital,Ministry of Health from December 2010to April *** sample was tested by RT-PCR and conventional culture-based method for the presence of *** The total number of the specimens was *** with the conventional culture-based method,we demonstrated the diagnostic values for Real-Time PCR were 96.4%sensitivity,96.6%specificity,81.8%positive predictive rate,and 99.4%negative predictive *** the limit of detection was 10~2CFU/*** This Real-Time PCR is a simple,rapid,sensitive and specific *** the high negative predictive value,it can be used for the exclusion of MRSA colonization or ***,the application of its low positive predictive value should be further evaluated.