Effect of exercise training intensity on adipose tissue hormone sensitive lipase gene expression in obese women under weight loss

作     者：Tongjian You Xuewen Wang Rongze Yang Mary F.Lyles Dawei Gong Barbara J.Nicklas 

作者机构：Department of Exercise and Health SciencesCollege of Nursing and Health SciencesUniversity of MassachusettsBostonMA 02125USA Department of Exercise ScienceUniversity of South CarolinaColumbiaSC 29208USA Division of EndocrinologyDiabetes and NutritionDepartment of MedicineUniversityof Maryland School of MedicineBaltimoreMD 21201USA Section on Gerontology and Geriatric MedicineDepartment of Internal MedicineWake Forest University School of MedicineWinston-SalemNC 27157USA Division of EndocrinologyDiabetes and NutritionDepartment of MedicineUniversity of Maryland School of MedicineBaltimoreMD 21201USA Center on Human GenomicsWake Forest University School of MedicineWinston-SalemNC 27157USA 

出 版 物：《Journal of Sport and Health Science》 (运动与健康科学（英文）)

年 卷 期：2012年第1卷第3期

页      面：184-190,195页

核心收录：

学科分类：0403[教育学-体育学] 040302[教育学-运动人体科学(可授教育学、理学、医学学位)] 04[教育学] 

基　　金：supported by NIH grants R01-AG/ DK20583 P30-AG21332 and M01-RR07122 

主　　题：Abdominal obesity Adipose tissue Diet Exercise intensity Hormone sensitive lipase Weight loss 

摘      要：Background:Hormone sensitive lipase(HSL) is an enzyme that regulates adipose tissue lipolysis and plays an important role in chronic exerciseinduced changes in adipose tissue *** purpose of this study was to determine whether aerobic exercise intensity influences abdominal adipose tissue HSL gene expression in obese women under weight loss. Methods:Thirty women(body mass index(BMI) = 33.0±0.7 kg/m^2,age = 58±1 years) completed one of three 20-week interventions: caloric restriction alone(CR only,n = 8),CR plus moderate-intensity exercise(CR + moderate-intensity,45%—50%heart rate reserve(HRR). 3 day/week,n = 9),or CR plus vigorous-intensity exercise(CR + vigorous-intensity,70%—75%HRR,3 day/week,n = 13).Each group had a similar prescribed energy deficit comprised of underfeeding alone(2800 kcal/week for CR only) or underfeeding(2400 kcal/week) plus exercise(400 kcal/week).Body composition and maximal aerobic capacity(VO_2max) were measured,and subcutaneous abdominal adipose tissue samples were collected before and after the *** tissue HSL gene expression was measured by real time reversetranscriptase polymerase chain reaction. Results:All three interventions reduced body weight,fat mass,percent *** waist to a similar degree(all p 0.01).In addition,all interventions did not change absolute VO_2 max,but increased relative VO_2 max(p 0.05 to P 0.01).Compared to *** CR only nor CR + moderate-intensity changed adipose tissue HSL gene expression,but CR + vigorous-intensity significantly increased adipose tissue HSL gene expression(p 0.01).The changes of HSL gene expression levels in the CR + vigorous-intensity group were significantly different from those in the CR only(p 0.05) and CR + moderate-intensity(p 0.01) *** the whole cohort,changes in adipose tissue HSL gene expression correlated positively to changes in absolute(r = 0.55,p 0.01) and relative(r = 0.32,p = 0.09) VO_2 max. Conclusion:These res