Characterization of stereostructure by X-ray and technology of extracting in combination hydrolysis in situ of acankoreanogenin from leaves of Acanthopanax gracilistylus ***

作     者：戴玲 刘向前 谢霞 刘恒言 

作者机构：Hunan Key Laboratory of Traditional Chinese Medicine ModernizationSchool of PharmacyHunan University of Chinese Medicine 

出 版 物：《Journal of Central South University》 (中南大学学报（英文版）)

年 卷 期：2014年第21卷第8期

页      面：3063-3070页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 07[理学] 070205[理学-凝聚态物理] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 100602[医学-中西医结合临床] 10[医学] 0702[理学-物理学] 

基　　金：Project(11JJ2042)supported by the Natural Science Foundation of Hunan Province,China Project supported by the "Twelfth Five-Year" Key Discipline of Hunan University of Chinese Medicine-Pharmaceutical Analysis Science,China Project(11K048)supported by the Innovation Platform and Open Foundation Program of Higher Colleges of Hunan Province,China Project(K1207010-21)supported by the Changsha City Science and Technology Bureau Key Projects,China 

主　　题：acankoreanogenin extraction in combination hydrolysis in situ Acanthopanax gracilistylus W. W. Smith (AGS) centralcomposite design-response surface methodology 

摘      要：To study the stereostructure by X-ray and the technology of extracting acankoreanogenin from the leaves of Acanthopanax graeilistylus W. W. Smith （AGS）, the crystal structure was measured with a Bruker APEX-Ⅱ area-detector diffractometer instrument and the technology of extracting in combination hydrolysis in situ （ECHS） was compared with these of traditional methods. The crystal belongs to the monoclinic system, space group P2b with unit cell parameters： a=（8.3652±0.0006） nm, b=（24.721±0.002） nm, and c=（14.5587±0.0011） nm, α=90°, β=97.850 （4） °, γ=90 °, V=2982.51 nm3, Dc= 1.179 mg/m3, and the molecular number （Z） of elementary structures was 2. The comparisons show that the extraction rate of acankoreanogenin with ECHS methods is much higher than that of traditional methods. Then, central composite design-response surface methodology （CCD-RSM） was adopted for optimizing the extraction rate of ECHS methods. The optimized values of extraction parameters are as follows： for the for extraction process of acid hydrolysis are that extraction time 110.8 min, solvent-herb ratio 11.5 and acid content 5.25%; the best extraction process of basic hydrolysis are that extract time 120 min, solvent-herb ratio 8.7 and the alkali content 8.79%. Finally, the extracts were purified with decolorizing carbon after alkali solution and acid-isolation and purity of acankoreanogenin was 98.7%.