Effect on serum metabolomics of rats with premature ovarian insufficiency by Zhibian(BL54) through Shuidao(ST28) acupuncture

作     者：YAN Jing FENG Huimin QIU Fang WANG Haijun YIN Luyun JIN Xiaofei ZHAO Jiyu WANG Hongyang YAN Xiaoqin 

作者机构：Second Clinical College of Shanxi University of Traditional Chinese MedicineJinzhong 030619China Research Center of Experimental Acupuncture ScienceTianjin University of Traditional Chinese MedicineTianjin 301617China Department of Cervical and Lumbar Vertebral DiseasesJinzhong Hospital Affiliated to Shanxi University of Traditional Chinese MedicineJinzhong 030600China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2024年第44卷第4期

页      面：722-733页

核心收录：

学科分类：1005[医学-中医学] 100512[医学-针灸推拿学] 10[医学] 

基　　金：the Research Grant from the Natural Science Research Program of Shanxi Province:Based on Ultra-Performance Liquid Chromatography-Mass Spectrometry the effect of"Zhibian(BL54)through Shuidao(ST28)"Needle Method on Ovarian Metabolism and Apoptosis Pathways in rats with Premature Ovarian Insufficiency was Discussed(No.2022030211216) 

主　　题：acupuncture premature ovarian insufficiency metabolomics ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry Zhibian(BL54)through Shuidao(ST28) 

摘      要：OBJECTIVE:To analyze the serum metabolic targets of theZhibian(BL54)through Shuidao(ST28)acupuncture technique in cyclophosphamide(CTX)-induced premature ovarian insufficiency(POI)model rats and to elucidate the potential molecular mechanism of acupuncture in improving ***:We used an intraperitoneal injection of CTX to establish the POI rat model(POI group)and compared serum hormone levels and ovarian histopathological changes to evaluate the effect of the Zhibian(BL54)through Shuidao(ST28)technique(ZS+POI group)on ovarian ***,nontargeted metabolomics was performed using rat serum by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF/MS).RESULTS:After acupuncture intervention,the serum hormone levels and ovarian pathological morphology of POI rats were effectively ***,UPLC-Q-TOF/MS results showed that the ZS+POI group showed a significant reversal of the levels of 6 differential *** them,the levels of four serum metabolic markers,divanillyltetrahydrofuran ferulate,trans-ferulic acid,tryptamine,and neuraminic acid,increased *** analysis of biological effects showed that all metabolites were involved in the regulation of reproductive hormone levels and antioxidant and antiapoptotic ***:TheZhibian(BL54)through Shuidao(ST28)acupuncture method may improve the ovarian function of POI rats by regulating serum metabolite markers to exert antioxidant and antiapoptotic effects,which provides a theoretical basis for the clinical application of acupuncture in the treatment of POI.