Protective Effects of Berberine on Nonalcoholic Fatty Liver Disease in db/db Mice via AMPK/SIRT1 Pathway Activation

作     者：Cheng CHEN Xiao-cui LIU Bin DENG Cheng CHEN;Xiao-cui LIU;Bin DENG

作者机构：Jingzhou Hospital Affiliated to Yangtze UniversityJingzhou434020China Department of PharmacyUnion HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China State Key Laboratory of Phytochemistry and Plant Resources in West ChinaKunming Institute of BotanyChinese Academy of SciencesKunming650201China University of Chinese Academy of SciencesBeijing100049China 

出 版 物：《Current Medical Science》 (当代医学科学（英文）)

年 卷 期：2024年第44卷第5期

页      面：902-911页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by grants from the National Natural Science Foundation of China(No.81803799) Hubei Province Natural Science Foundation of China(No.2022CFB092) 

主　　题：nonalcoholic fatty liver disease berberine inflammation oxidative stress apoptosis 

摘      要：Objective Berberine(BBR)has emerged as a promising therapeutic agent for nonalcoholic fatty liver disease(NAFLD).This study aims to elucidate the underlying molecular *** In this study,db/db mice were chosen as an animal model for NAFLD.A total of 10 healthy C57BL/6J mice and 30 db/db mice were randomly allocated to one of 4 groups:the normal control(NC)group,the diabetic control(DC)group,the Metformin(MET)therapy group,and the BBR therapy *** total cholesterol(TC),triacylglycerol(TG),low-density lipoprotein cholesterol(LDL-c),high-density lipoprotein cholesterol(HDL-c),aspartate aminotransferase(AST)and alanine aminotransferase(ALT)levels in the serum were *** glutathione peroxidase(GSH-Px),glutathione(GSH),malondialdehyde(MDA),superoxide dismutase(SOD),catalase(CAT),interleukin(IL)-1β,tumor necrosis factor(TNF)-αand monocyte chemotactic protein 1(MCP-1)levels in liver tissue were *** and eosin(H&E),acid-Schiff(PAS)and TUNEL stanning was performed for histopathological *** blotting and immunohistochemistry were conducted to detect the expression levels of key proteins in the AMPK/SIRT1 *** BBR could improve lipid metabolism,attenuate hepatic steatosis and alleviate liver injury *** excessive oxidative stress,high levels of inflammation and abnormal apoptosis in db/db mice were reversed after BBR *** clearly changed the expression of AMP-activated protein kinase(AMPK)/Sirtuin 1(SIRT1),and their downstream *** BBR could reverse NAFLD-related liver injury,likely by activating the AMPK/SIRT1 signaling pathway to inhibit oxidative stress,inflammation and apoptosis in hepatic tissue.