Effects of gravity,microgravity or microgravity simulation on early mouse embryogenesis:A review of the first two space embryo studies

作     者：Douglas M.Ruden Daniel A.Rappolee 

作者机构：s.mott center for human health and developmentdepartment of obstetrics and gynecologywayne state universitydetroitmiunited states institute of environmental health scienceswayne state universitydetroitmiunited states 

出 版 物：《Mechanobiology in Medicine》 (力学生物学与医学（英文）)

年 卷 期：2024年第2卷第4期

页      面：7-14页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by an NIH ECHO grant to DMR UG3 OD023285 

主　　题：Microgravity Clinostat International space station(ISS) Embryogenesis 

摘      要：Many simulated micro-gravity(micro-G)experiments on earth suggest that micro-G conditions are not compatible with early mammalian embryo ***,the first two“space embryostudies have been published showing that early mouse embryo development can occur in real microgravity(real micro-G)conditions in *** the first of these studies,published in 2020,Lei and collaborators developed automated mini-incubator(AMI)devices for mouse embryos facilitating cultivation,microscopic observation,and *** these AMI apparatuses,3400 non-frozen 2-cell embryos were launched in a recoverable satellite,experiencing sustained microgravity(~0.001G)for 64 h post-orbit before fixation in space and recovery on *** a subsequent study,in 2023,Wakayama and colleagues2 devised Embryo Thawing and Culturing(ETC)devices,enabling manual thawing,cultivation,and fixation of frozen 2-cell mouse embryos by a trained astronaut aboard the International Space Station(ISS).Within the ETCs,a total of 7202-cell mouse embryos underwent thawing and cultivation for 4 days on the ISS,subject to either microgravity(n?360)and simulated-1G(n?360)*** primary findings from both space embryo experiments indicate that mouse embryos can progress through embryogenesis from the 2-cell stage to the blastocyst stage under real micro-G conditions with few ***,these studies propose the potential for mammalian reproduction under real micro-G conditions,challenging earlier simulated micro-G research suggesting otherwise.