Integrated photothermal microcarriers for precise exosome-secreted microRNA profiling in breast cancer diagnosis

作     者：Yunjie Shi Yun Cheng Peiyu Chen Lexiang Zhang Fangfu Ye Yunjie Shi;Yun Cheng;Peiyu Chen;Lexiang Zhang;Fangfu Ye

作者机构：School of Pharmaceutical SciencesWenzhou Medical UniversityWenzhouChina Oujiang Laboratory(Zhejiang Lab for Regenerative MedicineVision and Brain Health)Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhouChina Department of PediatricsThe Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhouChina Joint Centre of Translational Medicinethe First Affiliated Hospital of Wenzhou Medical UniversityWenzhouChina Beijing National Laboratory for Condensed Matter PhysicsInstitute of PhysicsChinese Academy of SciencesBeijingChina 

出 版 物：《Quantitative Biology》 (定量生物学（英文版）)

年 卷 期：2024年第12卷第4期

页      面：389-399页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0711[理学-系统科学] 1002[医学-临床医学] 07[理学] 08[工学] 100214[医学-肿瘤学] 10[医学] 

基　　金：National Key Research and Development Program of China,Grant/Award Number:2022YFA1405002 National Natural Science Foundation of China,Grant/Award Numbers:12325405,22307098 Wenzhou Institute,University of Chinese Academy of Sciences,Grant/Award Number:WIUCASQD2021012 Wenzhou high‐level innovation team Development and application team of functional liv Key Projects of Wenzhou Science and Technology Bureau,Grant/Award Number:ZG2023013 Wenzhou Basic Research Projects,Grant/Award Number:Y2023147 Zhejiang Provincial Natural Science Foundation of China,Grant/Award Number:LGF22C100003 Key Laboratory of Structural Malformations in Children of Zhejiang Province,Grant/Award Number:ZJET2301Z 

主　　题：breast cancer digital PCR exosomes MicroRNA photothermal 

摘      要：Breast cancer constitutes a significant global health burden,while conventional diagnosis approaches may lack precision and can be discomforting for *** have emerged as promising biomarkers for breast cancer due to their participation in diverse pathological processes,and a convenient analysis platform is believed to greatly promote its *** this study,we propose a novel digital PCR approach utilizing near-infrared(NIR)photo-responsive thermosensitive microcarriers integrated with black phosphorus for quantifying microRNA(miRNA)biomarkers within ***-like biomimetic nanomaterials were firstly assembled for nonspecific exosome capture based on the affinity effect of avidin and ***-responsive microcarriers,fabricated using gelatin-based substrates blended with photothermal nanocomposite,exhibited NIRinduced heating and reversible phase transition *** optimized synthesis parameters on thermal response and established a programmable and controllable NIR light source *** results indicated a significant elevation in the levels of biomarkers miRNA-1246 and miRNA-122,with fold increases ranging from 6.2 to 23.6 and 5.9 to 13.0,respectively,in breast cancer cell lines MCF-7 and MDA-MB-231 compared to healthy control cells *** study offers broad prospects for utilizing exosomes to resolve predictive biomarkers.