Ethylene Production and 1-Aminocyclopropane-l-Carboxylate （ACC） Synthase Gene Expression in Tomato （ L ycopsicon esculentum Mill.） Leaves Under Enhanced UV-B Radiation

作     者：Lizhe An Xiaofeng Xu Hongguan Tang Manxiao Zhang Zongdong HOU Yanhong Liu Zhiguang Zhao Huyuan Feng Shijian Xu Xunling Wang 

作者机构：Key Laboratory of Arid and Grassland Ecology of Ministry of Education Lanzhou University Lanzhou 730000 China Cold and Arid Regions Environmental and Engineering Research Institute the Chinese Academy of Sciences Lanzhou 730000 China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2006年第48卷第10期

页      面：1190-1196页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基　　金：Supported by the National Natural Science Foundation of China (30170038  90302010)  "Hundred talents" project of the Chinese Academy of Sciences  Gansu Key Technologies R&D Program (GS022-A41-045)  and Gansu Agricultural Bio-technology Research & Development Project. Acknowledgements The authors thank Dr Akira Nakatsuka (Laboratory of Postharvest Agriculture  Faculty of Agriculture  0kayama University  0kayama  Japan) for providing the ACC synthase clone 

主　　题：enhanced UV-B radiation ethylene production gene expression of 1-aminocyclopropane-l-carboxylate (ACC) synthase Lycopsicon esculentum 

摘      要：Tomato （Lycopslcon esculentum Mill.） plants grown in a greenhouse were irradiated with two different levels of UV-B, namely 8.82 （T1） and 12.6 kJ/m^2 per day （T2）. Ethylene production, 1-aminocyclopropane-1-carboxylate （ACC） content, 1-（malonylamino） cyclopvopane-1-carboxylic acid （MACC） content, gene expression of ACC aynthase （EC 4.***.1.14）, and ACC oxidase activity in tomato leaves were determined. The results Indicated that ACC content, the activity of ACC synthase and ACC oxidase, and ethylene production Increased continuously under low doses of UV-B radiation, whereas at high doses of radiation these parameters Increased during the first 12 d and then started to decrease. The MACC content increased continuously over 18 d under both doses of UV-B irradiation. The changes in ACC content, ACC synthaae activity, ACC oxidase activity, the transcriptional level of the ACC synthase gene, and ethylene production were consistent with each other, suggesting that ACC synthase was the key enzyme in ethylene biosynthesis and that ethylene production in tomato leaf tissues under UV-B radiation could be regulated by the expression of the ACC synthase gene. The results also indicate that the change in ethylene metabolism may be an adaptive mechanism to enhanced UV-B radiation.