DNA vaccination using bacillus Calmette-Guerin-DNA as an adjuvant to enhance immune response to three kinds of swine diseases

作     者：Zhang S Guo YJ Sun SH Wang KY Wang KH Zhang Y Zhu WJ Chen ZH Jiang L 

作者机构：Department of Medical Genetics College of Basic Medical SciencesSecond Military Medical University Shanghai 200433 China 

出 版 物：《第二军医大学学报》 (Academic Journal of Second Military Medical University)

年 卷 期：2006年第27卷第6期

页      面：606-606页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

主　　题：DNA疫苗 免疫反应 疾病 预防 

摘      要：In order to enhance the immune efficacy of DNA vaccination, experiments were conducted to investigate the regulating effects of Bacillus Calmette-Guerin (BCG)-DNA as an adjuvant on immune responses of mice against foot-and-mouth disease (FMD), Aujeszky s disease (AID) and classical swine fever (CSF)。BCG-DNA was purified from BCG by ion-exchange chromatography。Three DNA vaccines (pVSG, pVgD and pVE2) against the respective infection were constructed, and BCGDNA was coimmunized to mice by muscle injection。The results showed that titres of specific immunoglobulin (Ig)G to the vaccines mounted remarkably in the sera of the adjuvant covaccinated mice (P 0.05)。These results suggest that codelivery of BCG-DNA with DNA vaccines against FMD, AjD and CSF can enhance the induction of antigen-specific, especially, cell-mediated immunity。