Heyingwuzi formulation alleviates diabetic retinopathy by promoting mitophagy via the HIF-1α/BNIP3/NIX axis

作     者：Jia-Jun Wu Shu-Yan Zhang Lin Mu Zhi-Guo Dong Yin-Jian Zhang 

作者机构：Department of OphthalmologyLonghua Hospital Shanghai University of Traditional Chinese MedicineShanghai 200032China Department of OphthalmologyEye and ENT HospitalFudan UniversityShanghai 200031China 

出 版 物：《World Journal of Diabetes》 (世界糖尿病杂志（英文版）（电子版）)

年 卷 期：2024年第15卷第6期

页      面：1317-1339页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 100212[医学-眼科学] 10[医学] 

基　　金：Supported by the National Key Research and Development Project of China,No.2019YFC1711605 National Natural Science Foundation of China,No.81904257 Medical Innovation Research Project of Science and Technology Commission of Shanghai Municipality,No.21Y11923100 

主　　题：Diabetic retinopathy HIF-1α/BNIP3/NIX axis Mitophagy Heyingwuzi formulation Traditional Chinese medicine 

摘      要：BACKGROUND Diabetic retinopathy(DR)is the primary cause of visual problems in patients with *** Heyingwuzi formulation(HYWZF)is effective against *** To determine the HYWZF prevention mechanisms,especially those underlying *** Human retinal capillary endothelial cells(HRCECs)were treated with high glucose(hg),HYWZF serum,PX-478,or Mdivi-1 in ***,cell counting kit-8,transwell,and tube formation assays were used to evaluate HRCEC proliferation,invasion,and tube formation,*** electron microscopy was used to assess mitochondrial morphology,and Western blotting was used to determine the protein *** cytometry was used to assess cell apoptosis,reactive oxygen species(ROS)production,and mitochondrial membrane ***,C57BL/6 mice were established in vivo using streptozotocin and treated with HYWZF for four *** glucose levels and body weight were monitored *** in retinal characteristics were evaluated using hematoxylin and eosin,tar violet,and periodic acid-Schiff *** levels in retinal tissues were determined via Western blotting,immunohistochemistry,and *** HYWZF inhibited excessive ROS production,apoptosis,tube formation,and invasion in hg-induced HRCECs via mitochondrial autophagy in *** increased the mRNA expression levels of BCL2-interacting protein 3(BNIP3),FUN14 domain-containing 1,BNIP3-like(BNIP3L,also known as NIX),PARKIN,PTEN-induced kinase 1,and hypoxia-inducible factor(HIF)-1α.Moreover,it downregulated the protein levels of vascular endothelial cell growth factor and increased the light chain 3-II/I ***,PX-478 and Mdivi-1 reversed these ***,PX-478 and Mdivi-1 rescued the effects of HYWZF by decreasing oxidative stress and apoptosis and increasing *** intervention improved the symptoms of diabetes,tissue damage,number of acellular capillaries,and oxidative stress in ***,in vivo