Therapeutic effects of Lingguizhugan decoction in a rat model of high-fat diet-induced insulin resistance

作     者：Xiao-Ming Liu Shi-Qing Yuan Ying Ning Shi-Jia Nie Xu-Qiong Wang Hong-Yi Jia Xiu-Li Zheng 

作者机构：Department of Basic MedicineSchool of Basic Medicine Chengdu University of Traditional Chinese MedicineChengdu 611137Sichuan ProvinceChina Department of Clinical MedicineChengdu University of Traditional Chinese MedicineChengdu 610072Sichuan ProvinceChina 

出 版 物：《World Journal of Diabetes》 (世界糖尿病杂志（英文版）（电子版）)

年 卷 期：2024年第15卷第6期

页      面：1291-1298页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：Supported by the Preresearch Project of the National Natural Science Foundation of China,No.ZRYY1906 the Applied Basic Research Project of the Science and Technology Department of Sichuan Province,No.2021YJ0154 the Talent Research Promotion Plan of Xinglin Scholars of Chengdu University of Traditional Chinese Medicine,No.QNXZ2019035 the Chengdu University of Traditional Chinese Medicine‘Xinglin Scholars'subject talent research promotion Program(young scholars),No.QNXZ2019037 

主　　题：Lingguizhugan decoction High-fat diet-induced insulin resistance Amino acid metabolism S6K1 

摘      要：BACKGROUND Lingguizhugan(LGZG)decoction is a widely used classic Chinese medicine formula that was recently shown to improve high-fat diet(HFD)-induced insulin resistance(IR)in animal *** To assess the therapeutic effect of LGZG decoction on HFD-induced IR and explore the potential underlying *** To establish an IR rat model,a 12-wk HFD was administered,followed by a 4-wk treatment with *** determination of IR status was achieved through the use of biochemical tests and oral glucose tolerance *** a targeted metabolomics platform to analyze changes in serum metabolites,quantitative real-time PCR(qRT-PCR)was used to assess the gene expression of the ribosomal protein S6 kinase beta 1(S6K1).RESULTS In IR rats,LGZG decreased body weight and indices of hepatic *** effectively controlled blood glucose and food intake while protecting islet *** analysis revealed significant differences between the HFD and HFDLGZG *** intervention reduced branched-chain amino acid *** of IR-related metabolites such as tryptophan,alanine,taurine,and asparagine decreased *** may be linked to amino acids due to the contemporaneous increase in S6K1 expression,as shown by *** Our study strongly suggests that LGZG decoction reduces HFD-induced *** may activate S6K1 via metabolic *** findings lay the groundwork for the potential of LGZG as an IR treatment.