TCM-HIN2Vec:A strategy for uncovering biological basis of heart qi deficiency pattern based on network embedding and transcriptomic experiment

作     者：Lihong Diao Xinyi Fan Jiang Yu Kai Huang Edouard C.Nice Chao Liu Dong Li Shuzhen Guo 

作者机构：School of Traditional Chinese MedicineBeijing University of Chinese MedicineBeijing 102488China Department of Biochemistry and Molecular BiologyMonash UniversityClayton VIC 3800Australia Beijing Institute of LifeomicsBeijing 102206China State Key Laboratory of Medical ProteomicsBeijing 102206China National Center for Protein Sciences(Beijing)Beijing 102206China Beijing Proteome Research CenterBeijing 102206China 

出 版 物：《Journal of Traditional Chinese Medical Sciences》 (中医科学杂志（英文）)

年 卷 期：2024年第11卷第3期

页      面：264-274页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(32088101) National key Research and Development Program of China(2017YFC1700105,2021YFA1301603) 

主　　题：Qi deficiency pattern Heart failure Biological basis Network embedding Transcriptome 

摘      要：Objective To elucidate the biological basis of the heart qi deficiency(HQD)pattern,an in-depth understanding of which is essential for improving clinical herbal *** We predicted and characterized HQD pattern genes using the new strategy,TCM-HIN2Vec,which involves heterogeneous network embedding and transcriptomic ***,a heterogeneous network of traditional Chinese medicine(TCM)patterns was constructed using public ***,we predicted HQD pattern genes using a heterogeneous network-embedding *** then analyzed the functional characteristics of HQD pattern genes using gene enrichment analysis and examined gene expression levels using ***,we identified TCM herbs that demonstrated enriched interactions with HQD pattern genes via herbal enrichment *** Our TCM-HIN2Vec strategy revealed that candidate genes associated with HQD pattern were significantly enriched in energy metabolism,signal transduction pathways,and immune ***,we found that these candidate genes were significantly differentially expressed in the transcriptional profile of mice model with heart failure with a qi deficiency ***,herbal enrichment analysis identified TCM herbs that demonstrated enriched interactions with the top 10 candidate genes and could potentially serve as drug candidates for treating *** Our results suggested that TCM-HIN2Vec is capable of not only accurately identifying HQD pattern genes,but also deciphering the basis of HQD *** our finding indicated that TCM-HIN2Vec may be further expanded to develop other patterns,leading to a new approach aimed at elucidating general TCM patterns and developing precision medicine.