Preparation of Ephedra houttuynia Granule and Its Therapeutic Mechanism of Anti-Mycoplasma gallisepticum Infection Based on Network Pharmacology

作     者：Zhang Huanqi Han Mingdong Miao Yusong Xu Tianqiao Liang Xin Li Jichang Zhang Huanqi;Han Mingdong;Miao Yusong;Xu Tianqiao;Liang Xin;Li Jichang

作者机构：Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical DevelopmentCollege of Veterinary MedicineNortheast Agricultural UniversityHarbin 150030China Heilongjiang Academy of Agricultural SciencesHarbin 150030China 

出 版 物：《Journal of Northeast Agricultural University(English Edition)》 (东北农业大学学报（英文版）)

年 卷 期：2024年第31卷第2期

页      面：76-85页

学科分类：0907[农学-林学] 090705[农学-野生动植物保护与利用] 09[农学] 

基　　金：the National Natural Science Foundation of China(32273062 31973005) 

主　　题：Mycoplasma galliscepticum therapeutic mechanism network pharmacology Ephedra houttuynia granule granulating process 

摘      要：The use of Chinese herbal medicines can replace antibiotics that cause drug-resistance problems,which are currently necessary for disease *** this paper,a traditional Chinese medicine compound named Ephedra houttuynia granule for the treatment of Mycoplasma galliscepticum(MG)infection was ***,its action mechanism was explored through network *** optimal extraction and granulation processes of the compound were determined by high performance liquid chromatography(HPLC)method and L9 orthogonal test,and in the treatment experiment,Ephedra houttuynia granule showed a significant therapeutic effect on MG *** the study of network pharmacology,the results showed that the core targets of Ephedra houttuynia granule against MG infection were vascular endothelial growth factor(VEGFA),fos proto-oncogene(FOS),prepro-coagulation factor II(F2),etc.,the gene ontology/kyoto encyclopedia of genes and genomes(GO/KEGG)analysis results indicated that the signaling pathways of neuroactive ligand receptor interaction,cAMP,IL-17,T cell receptor,and tumor necrosis factor(TNF)might involve in anti-MG *** conclusion,this study would provide a new idea for elucidating the action mechanism of other diseases in veterinary clinic,which had a certain guiding significance.