Astragalus membranaceus(Fisch.)*** by dissolving microneedles achieves systemic therapeutic effects at low doses Author links open overlay panel

作     者：Yiwen Chen Zihan Zhou Luzheng Zhang Zifan Ding Pengyue Li Cong Yan 

作者机构：School of Life SciencesBeijing University of Chinese MedicineBeijing 102488China Dongzhimen HospitalBeijing University of Chinese MedicineBeijing 100029China School of Chinese Materia MedicaBeijing University of Chinese MedicineBeijing 102488China 

出 版 物：《Journal of Traditional Chinese Medical Sciences》 (中医科学杂志（英文）)

年 卷 期：2024年第11卷第3期

页      面：340-350页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(82274225) NATCM's Project of High-level Construction of Key TCM Disciplines-Beijing University of Chinese Medicine-Life Science from the Perspective of Chinese Medicine(zyyzdxk-2023263) 

主　　题：Dissolving microneedle Astragaloside IV Astragalus polysaccharides In vivo diffusion Rheological properties Acupuncture Immunosuppression Drug delivery 

摘      要：Objective:To determine the main components of Astragalus membranaceus(Fisch.)Bge(***,Huang Qi),Astragaloside IV(AIV)and Astragalus polysaccharides(AP),to characterize their properties,evaluate their in vivo efficacy,and to analyze drug diffusion using dissolving microneedle(DMN)technology in ***: Respectively,AIV-and AP-loaded DMNs comprising chitosan(CTS)and polyvinyl alcohol(PVA)were prepared via dual-mold *** morphology,mechanical properties,in vivo solubility,and skin irritation characteristics were *** vivo efficacy was assessed in cyclophosphamide-induced immunosuppressed mice,in vivo diffusion of AIV and AP by DMNs and conventional methods was compared,and the rheological properties of AIV-CTS-PVA and AP-CTS-PVA mixtures were ***: Subcutaneous dissolution and absorption of AIV-CTS-PVA and AP-CTS-PVA microneedles(MNs)at low doses(50%–17%of intraperitoneal AIV injection and 12%–4%of intravenous AP injection)reduced the spleen index and acid phosphatase activity in immunosuppressed mouse models,increased the thymus index,and achieved equivalent or better systemic therapeutic *** with injections,AIV and AP achieved controllable solid-liquid conversion through delivery with CTS-PVA MNs,resulting in highly localized aggregation within 48 h,reducing the initial explosive effect of the drug,and achieving stable and slow drug ***: The present study enhances our understanding of the efficacy and remote effects of drug-loaded DMNs from a traditional Chinese medicine(TCM)perspective,thereby promoting the development of precise and efficient delivery of TCM and further expanding the drug-loading range and application scenarios for DMNs.