Tankyrase 2 promotes lung cancer cell malignancy

作     者：Ying Wang Yong-Jun Zhang 

作者机构：Department of Gynecological OncologyZhejiang Cancer HospitalHangzhou 310022Zhejiang ProvinceChina Department of Integrated Traditional Chinese and Western MedicineZhejiang Cancer HospitalHangzhou 310022Zhejiang ProvinceChina 

出 版 物：《World Journal of Clinical Oncology》 (世界临床肿瘤学杂志（英文版）)

年 卷 期：2024年第15卷第6期

页      面：755-764页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by Traditional Chinese Medicine Foundation of Zhejiang Province No.2019ZA020 

主　　题：Apoptosis Migration Lung Cancer Proliferation Tankyrase 2 

摘      要：BACKGROUND Tankyrase 2(TNKS2)is a potential candidate molecular target for the prognosis and treatment of non-small cell lung cancer(NSCLC),but its biological functions are *** To investigate the biological functions of TNKS2 in *** Using a lentiviral vector,we generated H647 model cells with TNKS2 knockdown by RNA interference and A549 model cells with TNKS2 overexpression by tran-sfection with a TNKS2 overexpressing *** and decreased exp-ression levels of TNKS2 in the two cell lines were verified using real-time reverse transcriptase-polymerase chain reaction and Western blot *** apopto-sis,proliferation,and migration were determined using flow cytometry,carbo-xyfluorescein succinimidyl ester staining,and scratch assay,***-munofluorescence staining was conducted to examine TNKS2 andβ-catenin ex-pression levels in the two transfected cell lines and the non-transfected *** TNKS2 mRNA and protein expression was significantly higher in the highly malignant NCI-H647 cells,while it remained at a low level in the less malignant A549 ***-mediated overexpression of TNKS2 in A549 cells resulted in a 3-fold increase in gene expression and a 1.7-fold increase in protein expression(P0.01).Conversely,shRNA interference targeting TNKS2 Led to an 8-fold decrease in gene expression and a 3-fold decrease in protein expression(P0.01)in NCI-H647 ***,the cell apoptosis rate was significantly reduced(50%)and cell migration rate was increased(35%)in the TNKS2 overexpression group than in the control group(P0.05).In contrast,shTNKS2 promoted apoptosis by more than one fold and reduced migration by 60%(P0.05).Immunofluorescence analysis revealed enhanced nuclear localization ofβ-catenin fluorescence signal associated with high TNKS2 expression *** blot analysis investigating TNKS2/β-catenin-related proteins indicated consistent changes between TNKS2 andβ-catenin expression in lung can