The NAC056 transcription factor confers freezing tolerance by positively regulating expression of CBFs and NIA1 in Arabidopsis

作     者：Peipei Xu Wei Ma Huafeng Feng Weiming Cai 

作者机构：Laboratory of Photosynthesis and EnvironmentCAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesNo.300 Fenglin RoadShanghai 200032China State Key Laboratory of NBC Protection for CivilianBeijing 102205China Department of Food ScienceCollege of Hospitality ManagementShanghai Business SchoolShanghai 200235China These authors contributed equally to this article 

出 版 物：《Plant Communications》 (植物通讯（英文）)

年 卷 期：2024年第5卷第7期

页      面：251-260页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：supported by the National Natural Science Foundation of China(grant nos.32371293,32171232,31500236,and 31570859) the Natural Science Foundation of Shanghai(grant no.22ZR1469500) 

主　　题：CBFs freezing tolerance NAC transcription factor nitrate assimilation root growth 

摘      要：Freezing stress can seriously affect plant growth and development,but the mechanisms of these effects and plant responses to freezing stress require further ***,we identified a NAM,ATAF1/2,and CUC2(NAC)-family transcription factor(TF),NAC056,that can promote freezing tolerance in ***056 mRNA levels are strongly induced by freezing stress in roots,and the nac056 mutant exhibits compromised freezing ***056 acts positively in response to freezing by directly promoting key C-repeat-binding factor(CBF)pathway ***,we found that CBF1 regulates nitrate assimilation by regulating the nitrate reductase gene NIA1 in plants;therefore,NAC056–CBF1–NIA1 form a regulatory module for the assimilation of nitrate and the growth of roots under freezing *** addition,35S::NAC056 transgenic plants show enhanced freezing tolerance,which is partially reversed in the cbfs triple ***,NAC056 confers freezing tolerance through the CBF pathway,mediating plant responses to balance growth and freezing stress tolerance.