SWIR FluorescenceImaging In Vivo Monitoring and Evaluating Implanted M2 Macrophages in Skeletal Muscle Regeneration

作     者：Mo Chen Yuzhou Chen Sijia Feng Shixian Dong Luyi Sun Huizhu Li Fuchun Chen Nguyen Thi Kim Thanh Yunxia Li Shiyi Chen You Wang Jun Chen Mo Chen;Yuzhou Chen;Sijia Feng;Shixian Dong;Luyi Sun;Huizhu Li;Fuchun Chen;Nguyen Thi Kim Thanh;Yunxia Li;Shiyi Chen;You Wang;Jun Chen

作者机构：Sports Medicine Institute of Fudan UniversityDepartment of Sports MedicineHuashan HospitalFudan UniversityShanghai 200040China Department of Bone and Joint SurgeryDepartment of OrthopedicsRenji HospitalSchool of MedicineShanghai Jiaotong UniversityShanghai 200127China Department of Anatomy and PhysiologySchool of MedicineShanghai Jiao Tong UniversityShanghai 200025China Key Laboratory of Infrared System Detection&Imaging TechnologyShanghai Institute of Technical PhysicsChinese Academy of SciencesShanghai 200083China Biophysics GroupDepartment of Physics and AstronomyUniversity College LondonLondon WC1E 6BTUK Healthcare Biomagnetic and Nanomaterials LaboratoriesUniversity College LondonLondon W1S 4BSUK 

出 版 物：《Engineering》 (工程（英文）)

年 卷 期：2024年第33卷第2期

页      面：283-294页

核心收录：

学科分类：0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 

基　　金：supported by Shanghai Sailing Program(22YF1438700) National Key Research and Development Program of China(2021YFA1201303) National Natural Science Foundation of China(82172511,81972121,81972129,82072521,82011530023,and 82111530200) Sanming Project of Medicine in Shenzhen(SZSM201612078) the Introduction Project of Clinical Medicine Expert Team for Suzhou(SZYJTD201714) Shanghai Talent Development Funding Scheme 2020080 Shanghai Sailing Program(21YF1404100 and 22YF1405200) Research Project of Shanghai Science and Technology Commission(22DZ2204900)。 

主　　题：In vivo Short-wave infrared Skeletal muscle Macrophage Regeneration 

摘      要：Skeletal muscle has a robust regeneration ability that is impaired by severe injury,disease,and aging.resulting in a decline in skeletal muscle function.Therefore,improving skeletal muscle regeneration is a key challenge in treating skeletal muscle-related disorders.Owing to their significant role in tissue regeneration,implantation of M2 macrophages(M2MФ)has great potential for improving skeletal muscle regeneration.Here,we present a short-wave infrared(SWIR)fluorescence imaging technique to obtain more in vivo information for an in-depth evaluation of the skeletal muscle regeneration effect after M2MФtransplantation.SWIR fluorescence imaging was employed to track implanted M2MФin the injured skeletal muscle of mouse models.It is found that the implanted M2MФaccumulated at the injury site for two weeks.Then,SWIR fluorescence imaging of blood vessels showed that M2MФimplantation could improve the relative perfusion ratio on day 5(1.09±0.09 vs 0.85±0.05;p=0.01)and day 9(1.38±0.16 vs 0.95±0.03;p=0.01)post-injury,as well as augment the degree of skeletal muscle regencration on day 13 post-injury.Finally,multiple linear regression analyses determined that post-injury time and relative perfusion ratio could be used as predictive indicators to evaluate skeletal muscle regeneration.These results provide more in vivo details about M2MФin skeletal muscle regeneration and confirm that M2MФcould promote angiogenesis and improve the degree of skeletal muscle repair,which will guide the research and development of M2MФimplantation to improve skeletal muscle regeneration.