The Bioinformatic Applications of Hi-C and Linked Reads

作     者：Libo Jiang Michael A.Quail Jack Fraser-Govil Haipeng Wang Xuequn Shi Karen Oliver Esther Mellado Gomez Fengtang Yang Zemin Ning 

作者机构：School of Life Sciences and MedicineShandong University of TechnologyZibo 255049China The Wellcome Sanger InstituteWellcome Genome CampusHinxtonCambridge CB101SAUK College of Food Science and TechnologyHainan UniversityHaikou 570228China 

出 版 物：《Genomics, Proteomics & Bioinformatics》 (基因组蛋白质组与生物信息学报（英文版）)

年 卷 期：2024年第22卷第4期

页      面：11-30页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：supported by from the National Natural Science Foundation of China(Grant No.32070601) the Natural Science Fund for Excellent Young Scholars of Shandong Province,China(Grant No.ZR2022YQ23) supported by the Wellcome Trust,UK(Grant No.WT206194) 

主　　题：Long-range NGS reads Hi-C Linked Reads Genome assembly Quality assessment. 

摘      要：Long-range sequencing grants insight into additional genetic information beyond what can be accessed by both short reads and modern longread *** new sequencing technologies,such as“Hi-Cand“Linked Reads,produce long-range datasets for high-throughput and high-resolution genome analyses,which are rapidly advancing the field of genome assembly,genome scaffolding,and more comprehensive variant *** this review,we focused on five major long-range sequencing technologies:high-throughput chromosome conformation capture(Hi-C),10X Genomics Linked Reads,haplotagging,transposase enzyme linked long-read sequencing(TELL-seq),and singletube long fragment read(stLFR).We detailed the mechanisms and data products of the five platforms and their important applications,evaluated the quality of sequencing data from different platforms,and discussed the currently available bioinformatics *** work will benefit the selection of appropriate long-range technology for specific biological studies.