Simultaneous genetic transformation and genome editing of mixed lines in soybean(Glycine max)and maize(Zea mays)
作者机构：Bayer Crop Science700 Chesterfield Parkway WChesterfieldMO 63017USA
出 版 物：《aBIOTECH》 (生物技术通报（英文版）)
年 卷 期：2024年第5卷第2期
页 面：169-183页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种]
基 金：The authors would like to express their gratitude to all their Bayer colleagues who contributed to this work from inception to reviewing this manuscript particularly the transformation production team for help in generating events precision genomics team for analyzing and characterizing the events Drs. Linda Rymarquis Annie Saltarikos Bala Karunanandaa Larry Gilbertson and David Wooten Peizhen Yang for valuable advice and leadership or support and Drs. Nathan Springer Jenn To Christine Shyu and Tom Slewinski for reviewing the manuscript
主 题：Maize Soybean Seed embryo explants TREDMIL Genetic transformation Genome editing
摘 要：Robust genome editing technologies are becoming part of the crop breeding ***,genome editing is usually conducted either at a single locus,or multiple loci,in a variety at one *** parallel genomics platforms,multifaceted genome editing capabilities,and flexible transformation systems enable targeted variation at nearly any locus,across the spectrum of genotypes within a *** demonstrate here the simultaneous transformation and editing of many genotypes,by targeting mixed seed embryo explants with genome editing machinery,followed by re-identification through genotyping after plant *** and Editing of Mixed Lines(TREDMIL)produced transformed individuals representing 101 of 104(97%)mixed elite genotypes in soybean;and 22 of 40(55%)and 9 of 36(25%)mixed maize female and male elite inbred genotypes,*** of edited genotypes for the regenerated individuals identified over 800 distinct edits at the Determinate1(Dt1)locus in samples from 101 soybean genotypes and 95 distinct Brown midrib3(Bm3)edits in samples from 17 maize *** results illustrate how TREDMIL can help accelerate the development and deployment of customized crop varieties for future precision breeding.
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