Recognition of the inducible,secretory small protein OsSSP1 by the membrane receptor OsSSR1 and the co-receptor OsBAK1 confers rice resistance to the blast fungus

作     者：Tianfeng Zhao Shijie Ma Ziying Kong Haimiao Zhang Yi Wang Junzhe Wang Jiazong Liu Wanzhen Feng Tong Liu Chunyan Liu Suochen Liang Shilin Lu Xinyu Li Haipeng Zhao Chongchong Lu Muhammad Zunair Latif Ziyi Yin Yang Li Xinhua Ding 

作者机构：State Key Laboratory of Crop BiologyShandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect PestsCollege of Plant ProtectionShandong Agricultural UniversityTaian 271018ShandongChina College of Breeding and Multiplication(Sanya Institute of Breeding and Multiplication)Hainan UniversitySanya 572024HainanChina 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2024年第17卷第5期

页      面：807-823页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 

基　　金：supported by the National Key R&D Program of China(2022YFD1401500 and 2022YFD1402100) the Nature Science Foundation of China(32202257,32272557,and 32072500) the Natural Science Foundation of Shandong Province(ZR2020MC117) the China Postdoctoral Science Foundation(2021M702027) the Major Basic Research Project of Natural Science Foundation of Shandong Province(ZR2022ZD23) the Taishan Scholar Program of Shandong Province(TSTP20221117) the Shandong Modern Agricultural Industry Technology System(SDAIT-04-08) and the Key Technology Research and Development Program of Shandong(2019JZZY020608). 

主　　题：rice plant apoplast OsssP1 receptor plant resistance Magnaporthe oryzae 

摘      要：The plant apoplast,which serves as the frontline battleground for long-term host–pathogen interactions,harbors a wealth of disease resistance resources.However,the identification of the disease resistance proteins in the apoplast is relatively lacking.In this study,we identified and characterized the rice secretory protein OsSSP1(Oryza sativa secretory small protein 1).OsSSP1 can be secreted into the plant apoplast,and either in vitro treatment of recombinant OsSSP1 or overexpression of OsSSP1 in rice could trigger plant immune response.The expression of OsSSP1 is suppressed significantly during Magnaporthe oryzae infection in the susceptible rice variety Taibei 309,and OsSSP1-overexpressing lines all show strong resistance to M.oryzae.Combining the knockout and overexpression results,we found that OsSSP1 positively regulates plant immunity in response to fungal infection.Moreover,the recognition and immune response triggered by OsSSP1 depend on an uncharacterized transmembrane OsSSR1(secretory small protein receptor 1)and the key co-receptor OsBAK1,since most of the induced immune response and resistance are lost in the absence of OsSSR1 or OsBAK1.Intriguingly,the OsSSP1 protein is relatively stable and can still induce plant resistance after 1 week of storage in the open environment,and exogenous OsSSP1 treatment for a 2-week period did not affect rice yield.Collectively,our study reveals that OsSSP1 can be secreted into the apoplast and percepted by OsSSR1 and OsBAK1 during fungal infection,thereby triggering the immune response to enhance plant resistance to M.oryzae.These findings provide novel resources and potential strategies for crop breeding and disease control.