Identification of transient receptor potential channel genes and functional characterization of TRPA1 in Spodoptera frugiperda

作     者：Yutong Zhang Hangwei Liu Song Cao Bin Li Yang Liu Guirong Wang Yutong Zhang;Hangwei Liu;Song Cao;Bin Li;Yang Liu;Guirong Wang

作者机构：State Key Laboratory for Biology of Plant Diseases and Insect PestsInstitute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Shenzhen BranchGuangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China Institute of Evolution and EcologySchool of Life SciencesCentral China Normal UniversityWuhan 430079China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2024年第23卷第6期

页      面：1994-2005页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090402[农学-农业昆虫与害虫防治] 

基　　金：funded by the Shenzhen Science and Technology Program,China(KQTD20180411143628272) the Special Funds for Science Technology Innovation and Industrial Development of Shenzhen Dapeng New District,China(pt202101-02) the National Key R&D Program of China(2022YFE0116500) 

主　　题：Spodoptera frugiperda transient receptor potential channel expression profile TRPA1 Xenopus oocyte 

摘      要：Spodoptera frugiperda is a highly destructive pest that has become a global problem due to its robust reproductive and migratory *** receptor potential(TRP)channels,which constitute a vast ion channel family,play pivotal roles in sensing the external environment and maintaining internal homeostasis in *** channels have been widely investigated for their critical roles in regulating various insect behaviors in recent *** this study,we identified 15 TRP gene loci encoding 26 transcripts in the genome of *** and analyzed their expression profiles at different developmental *** results revealed that *** possesses four TRPC genes,six TRPA genes,one TRPM gene,two TRPV genes,one TRPN gene,and one TRPML gene,while a canonical TRPP is ***,the SfruTRPA1 was functionally characterized using the Xenopus oocyte expression *** results showed that SfruTRPA1 is activated by temperature increases from 20 to 45℃,and there is no significant desensitization after repeated stimuli within the same temperature ***,SfruTRPA1 is activated by certain natural chemicals,including allyl isothiocyanate(AITC)and cinnamaldehyde(CA).These findings provide valuable insights to the TRP genes in ***.