UCHL1 promotes the proliferation of porcine granulosa cells by stabilizing CCNB1

作     者：Shengjie Shi Huan Yuan Lutong Zhang Lei Gao Lili Zhao Xiangfang Zeng Shiyan Qiao Guiyan Chu Chuanjiang Cai Shengjie Shi;Huan Yuan;Lutong Zhang;Lei Gao;Lili Zhao;Xiangfang Zeng;Shiyan Qiao;Guiyan Chu;Chuanjiang Cai

作者机构：College of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100ShaanxiChina State Key Laboratory of Animal NutritionMinistry of Agriculture Feed Industry CenterChina Agricultural UniversityBeijing 100193China 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2024年第15卷第5期

页      面：1894-1907页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：funded by National Key R&D Program of China(NO.2022YFD1300303) National Natural Science Foundation of China(32272849) 

主　　题：CCNB1 Granulosa cells Isovitexin Proliferation UCHL1 

摘      要：Background The proliferation of porcine ovarian granulosa cells(GCs)is essential to follicular development and the ubiquitin–proteasome system is necessary for maintaining cell cycle *** studies found that the deubiquitinase ubiquitin carboxyl-terminal hydrolase 1(UCHL1)regulates female reproduction,especially in ovarian ***,the mechanism by which UCHL1 regulates porcine GC proliferation remains *** UCHL1 overexpression promoted GC proliferation,and knockdown had the opposite ***1 is directly bound to cyclin B1(CCNB1),prolonging the half-life of CCNB1 and inhibiting its degradation,thereby promoting GC *** s more,a flavonoid compound-isovitexin improved the enzyme activity of UCHL1 and promoted the proliferation of porcine *** UCHL1 promoted the proliferation of porcine GCs by stabilizing CCNB1,and isovitexin enhanced the enzyme activity of *** findings reveal the role of UCHL1 and the potential of isovitexin in regulating proliferation and provide insights into identifying molecular markers and nutrients that affect follicle development.