Replacement of fish meal with cottonseed protein concentrate in Chinese mitten crab(Eriocheir sinensis): Nutrient digestibility, growth performance, free amino acid profile, and expression of genes related to nutrient metabolism
作者机构：Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring Institute of Modern Aquaculture Science and Engineering School of Life Science South China Normal University
出 版 物：《Animal Nutrition》 (动物营养(英文))
年 卷 期：2024年第17卷第2期
页 面：447-462页
核心收录：
学科分类：090801[农学-水产养殖] 0908[农学-水产] 09[农学]
基 金：funded by National Key R&D Program of China(2018YFD0900400) Guangzhou Science and Technology Planning Project (2023E04J1247)
主 题：Eriocheir sinensis Cottonseed protein concentrate Digestibility Amino acid TOR signaling pathway
摘 要：This study aimed to investigate the application of cottonseed protein concentrate(CPC) in Chinese mitten crabs(Eriocheir sinensis). First, the apparent digestibility coefficient(ADC) of CPC, fish meal and soybean meal were compared in crabs(21.72 ± 0.33 g). The protein ADC of CPC was 90.42%, which was significantly higher than that of soybean meal(83.16%)(P 0.05). The ADC of Phe, Cys and Glu of CPC were significantly higher than those of fish meal, while the ADC of Ile, Leu, Lys, Met, Thr and Ala of CPC were significantly lower(P 0.05). Second, we investigated the effects of fish meal substitution by CPC on growth performance, free amino acid profile, and expression of genes related to nutrient metabolism in crabs. Six diets were formulated by replacing 0%, 15%, 30%, 45%, 60% and 75% fish meal with CPC, namely FM, CPC15, CPC30, CPC45, CPC60, and CPC75. A total of 630 crabs(1.68 ± 0.00 g) were randomly divided into 18 tanks(3 tanks per group) and fed 3 times daily for 9 weeks. Results showed that CPC75 group significantly reduced growth performance, feed conversion efficiency, and free Ile, Leu, Lys, Met, and Thr contents in muscle(P 0.05). The contents of free amino acids(Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Val,Ala, Cys, Glu, Gly, Ser and Tyr) in hepatopancreas decreased linearly with the increase of dietary CPC level(P 0.05). The substitution of more than 45% fish meal with CPC significantly decreased the concentration of delicious amino acids(Ala, Glu and Gly) in hepatopancreas(P 0.05), which might adversely affect crab flavor. The expression of genes related to antioxidant capacity, protein transport, TOR pathway and lipid metabolism was significantly downregulated by increasing dietary CPC level(P 0.05). In conclusion, based on the quadratic regression analysis of FCR and PER, the optimal replacement levels of fish meal with CPC in crab diet containing 35% fish meal were 32.36% and 35.38%, respectively. It is recommended that Ile, Leu and Thr be
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