Inhibition of epidermal growth factor receptor expression by RNA interference in A549 cells

作     者：Min ZHANG, Xin ZHANG, Chun-xue BAI, Jie CHEN, MinQ WEILung Department of Zhong Shan Hospital, Fudan University, Shanghai 200032, China Department of Medicine, University of Queensland, Brisbane Qld 4032, Australia 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2004年第1期

页      面：63-69页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：ProjectsupportedbythegrantsfromScienceandTechnologyCommissionofShanghai(№02DJ14027) 

主　　题：epidermal growth factor receptor RNA interference double-stranded RNA small interfering RNA non-small-cell lung carcinoma 

摘      要：AIM: To investigate the biological features of A549 cells in which epidermal growth factor (EGF) receptors expression were suppressed by RNA interference (RNAi). METHODS: A549 cells were transfected using short small interfering RNAs (siRNAs) formulated with Lipofectamine 2000. The EGF receptor numbers were determined by Western blotting and flowcytometry. The antiproliferative effects of sequence specific double stranded RNA (dsRNA) were assessed using cell count, colony assay and scratch assay. The chemosensitivity of transfected cells to cisplatin was measured by MTT. RESULTS: Sequence specific dsRNA-EGFR down-regulated EGF receptor expression dramatically. Compared with the control group, dsRNA-EGFR reduced the cell number by 85.0 %, decreased the colonies by 63.3 %, inhibited the migration by 87.2 %, and increased the sensitivity of A549 to