OsNAC5 orchestrates OsABI5 to fine-tune cold tolerance in rice

作     者：Ruiqing Li Yue Song Xueqiang Wang Chenfan Zheng Bo Liu Huali Zhang Jian Ke Xuejing Wu Liquan Wu Ruifang Yang Meng Jiang 

作者机构：College of AgronomyAnhui Agricultural UniversityHefei 230036China Hainan InstituteYazhou Bay Sci-Tech CityZhejiang UniversitySanya 572025China National Key Laboratory of Rice BiologyAdvanced Seed InstituteZhejiang UniversityHangzhou 311225China State Key Laboratory of Rice Biology and Chinese National Center for Rice ImprovementChina National Rice Research InstituteHangzhou 311401China Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops(Co-Construction by Ministry and Province)Ministry of Agriculture and Rural AffairsCrop Breeding and Cultivation Research InstituteShanghai Academy of Agricultural SciencesShanghai 201106China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2024年第66卷第4期

页      面：660-682页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 

基　　金：supported by the National Natural Science Foundation of China(32071946 and 32201895) the Research Startup Funding from Hainan Institute of Zhejiang University(0201-6602-A12203) the“Nanhai New Star”Technology Innovation Talent Platform Project of Hainan Province(NHXXRCXM202362) the PhD Scientific Research and Innovation Foundation of Sanya Yazhou Bay Science and Technology City(HSPHDSRF-2023-04-018) the Fundamental Research Funds for the Central Universities(226-2022-00012) the Agriculture Research System of Shanghai,China(202203)。 

主　　题：ABA signaling ABI5 cold tolerance DREB NAC5 rice 

摘      要：Due to its tropical origins,rice(Oryza sativa)is susceptible to cold stress,which poses severe threats to production.OsNAC5,a NAC-type transcription factor,participates in the cold stress response of rice,but the detailed mechanisms remain poorly understood.Here,we demonstrate that OsNAC5 positively regulates cold tolerance at germination and in seedlings by directly activating the expression of ABSCISIC ACID INSENSITIVE 5(OsABI5).Haplotype analysis indicated that single nucleotide polymorphisms in a NAC-binding site in the OsABI5 promoter are strongly associated with cold tolerance.OsNAC5 also enhanced OsABI5 stability,thus regulating the expression of cold-responsive(COR)genes,enabling fine-tuned control of OsABI5 action for rapid,precise plant responses to cold stress.DNA affinity purification sequencing coupled with transcriptome deep sequencing identified several OsABI5 target genes involved in COR expression,including DEHYDRATION-RESPONSIVE ELEMENT BINDING FACTOR 1A(OsDREB1A),OsMYB20,and PEROXIDASE 70(OsPRX70).In vivo and in vitro analyses suggested that OsABI5 positively regulates COR gene transcription,with marked COR upregulation in OsNAC5-overexpressing lines and downregulation in osnac5 and/or osabi5 knockout mutants.This study extends our understanding of cold tolerance regulation via OsNAC5 through the OsABI5-CORs transcription module,which may be used to ameliorate cold tolerance in rice via advanced breeding.