scRNA-seq transcriptomic profiling of irradiated mouse skin reveals altered cell types,pathways,and cell-cell interactions

作     者：Zhisen Zhang Yinyin Shu Shuangshuang Lu Kai Kang Mintao Ji Peng Zhang Lei Chang 

作者机构：State Key Laboratory of Radiation Medicine and ProtectionSchool of Radiation Medicine and ProtectionCollaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education InstitutionsMedical College of Soochow UniversitySuzhou 215123China Pasteurien CollegeMedical College of Soochow UniversitySuzhou 215123China Department of OrthopaedicsThe Second Affiliated Hospital of Soochow UniversitySuzhou 215004China 

出 版 物：《Radiation Medicine and Protection》 (放射医学与防护（英文）)

年 卷 期：2024年第5卷第3期

页      面：185-193页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：National Natural Science Foundation of China(82373525,31971165,82173465) Leading Talents Program of Gusu District,China(ZXL2022454,LC) Jiangsu Provincial Outstanding Postdoctoral Program,China(2023ZB254,MT-J) Youth Fund of Jiangsu Provincial Natural Science Foundation,China(BK20230490,MT-J) 

主　　题：scRNA-seq IRIAD cell-cell interaction IL-17 

摘      要：Objective:To investigate the substantial changes in cell types,pathways,and cell-cell interactions occurring in the irradiation-induced alopecia and dermatitis(IRIAD)mouse model and to identify potential targets for patients experiencing skin adverse reactions to ***:Mice were irradiated at 15 Gy,targeting the head and neck *** a 14-day interval,living cells were extracted from both wild-type(WT)mice and irradiated mice for single-cell RNA sequencing(scRNA-seq).The scRNA-seq data,retrieved from the GEO database(GSE201447),underwent stringent quality control using the Seurat(v4.3.0)R *** type annotation relied on previously reported typical markers and CellMarker *** expressed genes were calculated to perform gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)***-cell interactions were evaluated using the Cellchat R ***:The application of single-cell RNA sequencing(scRNA-seq)enabled a comprehensive characterization of the intricate cellular composition of both wild-type(WT)and irradiated mice ***,cells within irradiated mice skin exhibited a significant alteration in the intensity of cell-cell interactions compared to their wild-type *** change in interaction intensity was observed across various cell types,including fibroblast cells,endothelial cells,and dendritic ***,theseinteracting cellsshared common signaling pathways,notably the upregulation of the IL-17 pathway following ***:The modification of intercellular communication induced by irradiation primarily involves fibroblast cells,endothelial cells,and various types of immune *** investigation provides a novel perspective on potential targets and holds promise for enhancing the clinical management of IRIAD.