The OsWRKY72-OsAAT30/OsGSTU26 module mediates reactive oxygen species scavenging to drive heterosis for salt tolerance in hybrid rice

作     者：Citao Liu Bigang Mao Yanxia Zhang Lei tian Biao Ma Zhuo Chen Zhongwei Wei Aifu Li Ye Shao Gongye Cheng Lingling Li Wenyu Li Di Zhang Xiaoping Ding Jiangxiang Peng Yulin Peng Jiwai He Nenghui Ye Dingyang Yuan Chengcai Chu Meijuan Duan 

作者机构：Hunan Provincial Key Laboratory of Stress BiologyCollege of AgricultureHunan Agricultural UniversityChangsha 410128China State Key Laboratory of Hybrid RiceHunan Hybrid Rice Research CenterChangsha 410125China College of AgricultureSouth China Agricultural UniversityGuangzhou 510642China State Key Laboratory of Wheat and Maize Crop ScienceCenter for Crop Genome EngineeringCollege of AgronomyHenan Agricultural UniversityZhengzhou 450002China College of HorticultureFujian Agriculture and Forestry UniversityFuzhou 350002China Department of Computational Medicine and BioinformaticsUniversity of MichiganAnn ArborMI 48109 USA Biobin Data Science Co.Ltd.Changsha 410221China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2024年第66卷第4期

页      面：709-730页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 

基　　金：supported by grants from the National Natural Science Foundation of China(Grant No.32272050 and U21A20208) the National Center of Technology Innovation for Saline-Alkali Tolerant Rice(2022PT1005) the Hunan Natural Science Foundation(Grant No.2022JJ30021) the Science and Technology Innovation Program of Hunan Province(2023NK1010) the Changsha Science and Technology Project(Grant No.kq2202221)。 

主　　题：allele-specific gene expression(ASE) heterosis hybrid rice nonadditive effect(NAE) salt tolerance transcriptome and proteome 

摘      要：Hybrid rice(Oryza sativa)generally outperforms its inbred parents in yield and stress tolerance,a phenomenon termed heterosis,but the underlying mechanism is not completely understood.Here,we combined transcriptome,proteome,physiological,and heterosis analyses to examine the salt response of super hybrid rice Chaoyou1000(CY1000).In addition to surpassing the mean values for its two parents(mid-parent heterosis),CY1000 exhibited a higher reactive oxygen species scavenging ability than both its parents(over-parent heterosis or heterobeltiosis).Nonadditive expression and allele-specific gene expression assays showed that the glutathione S-transferase gene OsGSTU26 and the amino acid transporter gene OsAAT30 may have major roles in heterosis for salt tolerance,acting in an overdominant fashion in CY1000.Furthermore,we identified OsWRKY72 as a common transcription factor that binds and regulates OsGSTU26 and OsAAT30.The salt-sensitive phenotypes were associated with the OsWRKY72^(paternal)genotype or the OsAAT30^(maternal)genotype in core rice germplasm varieties.OsWRKY72^(paternal)specifically repressed the expression of OsGSTU26 under salt stress,leading to salinity sensitivity,while OsWRKY72^(maternal)specifically repressed OsAAT30,resulting in salinity tolerance.These results suggest that the OsWRKY72-OsAAT30/OsGSTU26 module may play an important role in heterosis for salt tolerance in an overdominant fashion in CY1000 hybrid rice,providing valuable clues to elucidate the mechanism of heterosis for salinity tolerance in hybrid rice.