OsWRKY78 regulates panicle exsertion via gibberellin signaling pathway in rice

作     者：Enyang Mei Mingliang He Min Xu Jiaqi Tang Jiali Liu Yingxiang Liu Zhipeng Hong Xiufeng Li Zhenyu Wang Qingjie Guan Xiaojie Tian Qingyun Bu 

作者机构：State Key Laboratory of Black Soils Conservation and UtilizationKey Laboratory of Soybean Molecular Design BreedingNortheast Institute of Geography and AgroecologyChinese Academy of SciencesHarbin 150081China University of Chinese Academy of SciencesBeijing 100049China Key Laboratory of Saline-alkali Vegetation Ecology RestorationNortheast Forestry UniversityMinistry of EducationHarbin 150040China College of Life ScienceNortheast Forestry UniversityHarbin 150040China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2024年第66卷第4期

页      面：771-786页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 

基　　金：supported by the National Natural Science Foundation of China(Grant No.31671653,31801017) Heilongjiang Key Research and Development Program(Grant No.2022ZX02B03) National Natural Science Foundation of China-Heilongjiang Joint Fund(Grant No.U23A20193) Youth Innovation Promotion Association Chinese Academy of Sciences(Grant No.2021229) Young Scientist Group Project of Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences(Grant No.2023QNXZ02)。 

主　　题：gibberellin MAPK cascades OsWRKY78 panicle exsertion rice 

摘      要：Panicle exsertion is one of the crucial agronomic traits in rice(Oryza sativa).Shortening of panicle exsertion often leads to panicle enclosure and severely reduces seed production.Gibberellin(GA)plays important roles in regulating panicle exsertion.However,the underlying mechanism and the relative regulatory network remain elusive.Here,we characterized the oswrky78 mutant showing severe panicle enclosure,and found that the defect of oswrky78 is caused by decreased bioactive GA contents.Biochemical analysis demonstrates that OsWRKY78 can directly activate GA biosynthesis and indirectly suppress GA metabolism.Moreover,we found OsWRKY78 can interact with and be phosphorylated by mitogen-activated protein kinase(MAPK)kinase OsMAPK6,and this phosphorylation can enhance OsWRKY78 stability and is necessary for its biological function.Taken together,these results not only reveal the critical function of OsWRKY78,but also reveal its mechanism via mediating crosstalk between MAPK and the GA signaling pathway in regulating panicle exsertion.