Changes of evoked potential in different hippocampal regions induced by electrostimulation at medial mamillary nucleus of rats

作     者：Xinxin Li Lihong Shang Liang Zhang Fengzhi Cui 

作者机构：Department of Physiology Shenyang Medical College Shenyang 110034 Liaoning Province China Department of Physiology. Sujiatun Distdct Health School Shenyang 110101. Liaoning Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2006年第1卷第1期

页      面：87-89页

核心收录：

学科分类：10[医学] 

主　　题：Changes of evoked potential in different hippocampal regions induced by electrostimulation at medial mamillary nucleus of rats body 

摘      要：BACKGROUND： Morphological data have shown that the most important afferent fibers of papillary body come from hippocampal structure. OBJECTIVE： To observe the changes of evoked potential in hippocampus and the significance after electrostimulation at medial mamillary nucleus. DESIGN： An observational control experiment. SETTING： Department of Physiology, Shenyang Medical College. MATERIALS ： Twenty-three male or female Wistar rats, 3-4 months old, weighing 270-350 g, were provided by bhe animal room of Shenyang Medical College [the license number was scxk（Liao）2003-0016]. METHODS： The Wistar rats were anaesthetized by intraperintoneal injection of 20% urethane （1 g/kg）, tracheal intubation was also given. The self-made double-pole metal stimulating electrode with the point diameter of 1 mm was inserted into medial mamillary nucleus, the wanted hippocampal guidance spot was found within the rang of the hippocampal region at the same side of tee mamillary body range （CA1-CA4）, inserted with same-core guidance electrode, a sole square-wave stimulation of wave wide 0.2 ms stimulated with electrodes at the applied intensity of 7-9 V, the evoked potential was induced through guidance electrodes, and then input to the ATAC-350 data-processing machine for memory showing wave processing, the memory recorded wave recording graph was separately drawn up by the X-Y recording instrument to observe the latency, time procedure and amplitude of the evoked potential in each hippocampal region of the rats and calculate the percentage of the evoked potential in each hippocampal region. Totally 78 guidance spots in hippocampus were recorded, including 30 positive reaction spots and 48 negative ones. MAIN OUTCOME MEASURES： ① Latency, time procedure and amplitude of the evoked potentials in each hippocampal region of rats; ②percentage of the evoked potentials in each hippocampal region; ③ the wave shapes of the evoked potentials in each hippocampal region from different arrangem