Potential of guar gum as a leaky gut model in broilers:Digestibility,performance,and microbiota responses

作     者：Amine Mellouk Tahir Mahmood Maamer Jlali Nuria Vieco-Saiz Virginie Michel Pierre Cozannet Sarper Ozbek Yves Mercier Estelle Devillard Jessika Consuegra Amine Mellouk;Tahir Mahmood;Maamer Jlali;Nuria Vieco-Saiz;Virginie Michel;Pierre Cozannet;Sarper Ozbek;Yves Mercier;Estelle Devillard;Jessika Consuegra

作者机构：Department of R&I in Monogastric Animal NutritionEuropean Laboratory of Innovation Science & Expertise(ELISE)Adisseo France S.A.S.69 190Saint FonsFrance 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2024年第17卷第2期

页      面：177-187页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 

基　　金：Adisseo's Center of Expertise and Research in Nutrition CERN 

主　　题：Animal resilience Guar gum Broilers'gut health Performance Digestibility Microbiota 

摘      要：Diet is a major modulator of animal resilience and its three pillars:host s immune response,gut microbiota,and intestinal *** the present study,we endeavour to delineate a challenging con-dition aimed to degrade these pillars and elucidate its impact on broiler performance and nutrient *** attain this objective,we opted to use guar gum(GG)as a source of galactomannan.A series of three in vivo experiments were conducted employing conventional or semi-purified diets,supple-mented with or without GG during the grower phase(14-28 d).Our findings demonstrate a substantial decline in animal performance metrics such as body weight(reduced by 29%,P0.001),feed intake(decreased by 12%,P0.001),and feed conversion ratio(up to 58%increase,P0.001)in the presence of GG at 2%.The supplementation of a semi-purified diet with incremental doses of GG resulted in a linear reduction(P0.001)in the apparent total tract digestibility of dry matter and apparent metabolisable ***,a marked reduction in ileal endogenous losses,as well as apparent and standardised digestibility of all amino acids with varying proportions(P0.05),was *** alterations were accompanied by disrupted gut integrity assessed by fluorescein isothiocyanate-dextran(FITC-d)(P0.001)as well as an inflammatory status characterised by elevated levels of acute-phase proteins,namely orosomucoid and serum amyloid A in the sera(P=0.03),and increased mRNA expression levels of IL-1,IL-6,IL-8,Inos,and K203 genes in the ileum,along with a decrease in IgA levels in the gut lumen(P0.05).Microbial ecology and activity were characterised by reduced diversity and richness(Shannon index,P=0.005)in the presence of ***,our results revealed diminished levels of short-chain fatty acids(P=0.01)and their producer genera,such as Clostridium_XIVa and Blautia,in the gut caeca,coupled with excessive accumulation of lactate(17-fold increase,P0.01)in the presence of GG at 2%.In addition