The evolution,pathogenicity and transmissibility of quadruple reassortant H1N2 swine influenza virus in China:A potential threat to public health

作     者：Xinxin Cui Jinhuan Ma Zifeng Pang Lingzhi Chi Cuishan Mai Hanlin Liu Ming Liao Hailiang Sun 

作者机构：College of Veterinary MedicineSouth China Agricultural UniversityGuangzhou510642China Key Laboratory of Zoonosis Control and Prevention of Guangdong ProvinceSouth China Agricultural UniversityGuangzhou510642China National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and ControlSouth China Agricultural UniversityGuangzhou510642China Shandong Vocational Animal Science and Veterinary CollegeWeifang261061China Institute of Animal HealthGuangdong Academy of Agricultural SciencesGuangzhou510640China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2024年第39卷第2期

页      面：205-217页

核心收录：

学科分类：0710[理学-生物学] 090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：supported by Special fund for scientific innovation strategy-construction of high level Academy of Agriculture Science-Distinguished Scholar(R2020PYJC001). 

主　　题：Swine influenza virus(SIV) H1N2 Evolution Replication Pathogenicity Zoonotic potential 

摘      要：Swine are regarded as“intermediate hostsor“mixing vesselsof influenza viruses,capable of generating strains with pandemic potential.From 2020 to 2021,we conducted surveillance on swine H1N2 influenza(swH1N2)viruses in swine farms located in Guangdong,Yunnan,and Guizhou provinces in southern China,as well as Henan and Shandong provinces in northern China.We systematically analyzed the evolution and pathogenicity of swH1N2 isolates,and characterized their replication and transmission abilities.The isolated viruses are quadruple reassortant H1N2 viruses containing genes from pdm/09 H1N1(PB2,PB1,PA and NP genes),triple-reassortant swine(NS gene),Eurasian Avian-like(HA and M genes),and recent human H3N2(NA gene)lineages.The NA,PB2,and NP of SW/188/20 and SW/198/20 show high gene similarities to A/Guangdong/Yue Fang277/2017(H3N2).The HA gene of swH1N2 exhibits a high evolutionary rate.The five swH1N2 isolates replicate efficiently in human,canine,and swine cells,as well as in the turbinate,trachea,and lungs of mice.A/swine/Shandong/198/2020 strain efficiently replicates in the respiratory tract of pigs and effectively transmitted among them.Collectively,these current swH1N2 viruses possess zoonotic potential,highlighting the need for strengthened surveillance of swH1N2 viruses.