Laboratory studies on the infectivity of human respiratory viruses:Experimental conditions,detections,and resistance to the atmospheric environment

作     者：Yaohao Hu Shuyi Peng Bojiang Su Tao Wang Juying Lin Wei Sun Xiaodong Hu Guohua Zhang Xinming Wang Ping'an Peng Xinhui Bi 

作者机构：State Key Laboratory of Organic Geochemistry and Guangdong Provincial Key Laboratory of Environmental Protection and Resources UtilizationGuangzhou Institute of GeochemistryChinese Academy of SciencesGuangzhou 510640China University of Chinese Academy of SciencesBeijing 100049China Guangdong-Hong Kong-Macao Joint Laboratory for Environmental Pollution and ControlGuangzhou 510640China 

出 版 物：《Fundamental Research》 (自然科学基础研究（英文版）)

年 卷 期：2024年第4卷第3期

页      面：471-483页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(42130611) Guangdong Foundation for Program of Science and Technology Research(2023B1212060049,2019B121205006) 

主　　题：Respiratory viruses Viral infectivity Laboratory simulation Atmospheric environmental condition Airborne transmission 

摘      要：The environmental stability of infectious viruses in the laboratory setting is crucial to the transmission potential of human respiratory *** experimental techniques or conditions used in studies over the past decades have led to diverse understandings and predictions for the stability of viral infectivity in the atmospheric *** this paper,we review the current knowledge on the effect of simulated atmospheric conditions on the infectivity of respiratory viruses,mainly focusing on influenza viruses and coronaviruses,including severe acute respiratory syndrome coronavirus 2 and Middle East respiratory syndrome ***,we summarize the impact of the experimental conditions on viral stability;these involve the methods of viral aerosol generation,storage during aging and collection,the virus types and strains,the suspension matrixes,the initial inoculum volumes and concentrations,and the drying ***,we summarize and discuss the detection methods of viral infectivity and their ***,we integrate the results from the reviewed studies to obtain an overall understanding of the effects of atmospheric environmental conditions on the decay of infectious viruses,especially aerosolized ***,this review highlights the knowledge gaps in predicting the ability of viruses to maintain infectivity during airborne transmission.