Glycyrrhiza uralensis flavonoids inhibit brain microglial cell TNF-α secretion,p-IκB expression,and increase brain-derived neurotropic factor(BDNF)secretion

作     者：Sangita P.Patil Changda Liu Joseph Alban Nan Yang Xiu-Min Li Sangita P.Patil;Changda Liu;Joseph Alban;Nan Yang;Xiu-Min Li

作者机构：Department of PediatricsDivision of Pediatric Allergy and ImmunologyJaffe Food Allergy InstituteCenter for Integrative Medicine for Allergies and WellnessIcahn School of Medicine at Mount Sinai1425 Madison AvenueBox 1089New YorkNY 10029-6574USA 

出 版 物：《Journal of Traditional Chinese Medical Sciences》 (中医科学杂志（英文）)

年 卷 期：2014年第1卷第1期

页      面：28-37页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：This work was supported by NIH/NCCAM center grants#P01 AT002647-0101“Center for Chinese Herbal Therapy(CHT)for Asthma” the Sean Parker Foundation for(ASHMI Active Compounds for Asthma Therapy) and the Winston Wolkoff Fund for Integrative Medicine for Allergies and Wellness to XM Li 

主　　题：Anti-asthma herbal medicine intervention Anxiety and asthma Flavonoids TNF-α BDNF 

摘      要：Objective:Asthma sufferers exhibit high prevalence of anxiety/*** tumor-necrosis factor-alpha(TNF-α)levels in peripheral system and central nervous system(CNS)are associated with anxiety/depression,whereas brain-derived neurotropic factor(BDNF)has anti-depressant *** anti-asthma herbal medicine intervention ASHMI inhibits peripheral TNF-αsecretion in an animal model of *** hypothesize that ASHMI and its compounds may have modulatory effects on CNS TNF-αand BDNF *** sought to determine the effect of ASHMI and individual herb constituents on brain microglial cell TNF-αproduction,and identify the active compounds that suppress TNF-αand increase ***:BV-2 mouse microglial cells were pre-treated with ASHMI or extracts of Ganoderma lucidum(***),Sophora flavescens Ait(***),and Glycyrrhiza uralensis Fischer(***),the herbal constituents in ASHMI,or individual compounds isolated from *** at different concentrations and then stimulated with ***-αlevels in culture supernatants were measured by *** effect of active compounds on NFkB signaling pathway and on BDNF production were determined by western blotting and ELISA,***:ASHMI produced dose-dependent inhibition of TNF-αsecretion by cultured-mousemicroglia BV2 *** the three herb extracts in ASHMI,only *** significantly and dosedependently inhibited TNF-α*** the 5 flavonoids isolated from ***,isoliquiritigeninwas themost *** suppression of TNF-αproduction was associated with attenuation of p-NF-kB expression,andwas accompanied by increased BDNF ***:ASHMI and its effective flavonoid,isoliquiritigenin,inhibited TNF-αproduction by LPS stimulatedmicroglial cells and elevated BDNF levels,which may prove to have anti-CNS inflammatory and anti-anxiety effects.