Optimization of Extraction Process for Total Flavonoids from Penthorum chinense Pursh and Comparison of Their Contents from Different Parts

作     者：Hongsheng LIU Jiqiang YAO Xue BAI Jianying KANG Yefei YUAN 

作者机构：Department of PharmacyLuzhou Hospital of Traditional Chinese MedicineLuzhou 646000China Luzhou Feixiang Pharmaceutical Technology Co.LtdLuzhou 646000China Department of PharmacySouthwest Medical UniversityLuzhou 646000China 

出 版 物：《Medicinal Plant》 (药用植物：英文版)

年 卷 期：2024年第15卷第2期

页      面：10-14页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by Key Research and Development Program of Sichuan Province(2022YFS0436) Natural Science Foundation of Sichuan Province(2022NSFSC1738) Science and Technology Planning Project of Luzhou City(2021-JYJ-109,2023SYF120) Special Project of Traditional Chinese Medicine Scientific Research of Sichuan Provincial Administration of Traditional Chinese Medicine(2020CP0029) Southwest Medical University-Luzhou Hospital of Traditional Chinese Medicine Base Project(2019-LH003) Open Subject of Luzhou Key Laboratory of Fine Chemical Application Technology(HYJY-2106-B) Southwest Medical University Undergraduate Student Innovation and Entrepreneurship Training Program(202310632074) 

主　　题：Penthorum chinense Pursh Total flavonoids Orthogonal experiments Extraction process Content determination 

摘      要：[Objectives]This study was conducted to optimize the extraction process of total flavonoids from Penthorum chinense Pursh and compare their contents from different parts.[Methods]Single factor and orthogonal experiments were designed to optimize the extraction process of total flavonoids from *** Pursh with the volume fraction of ethanol,the ratio of material to liquid,heating reflux extraction time and extraction times as factors,and the content of total flavonoids as the index.A verification test was carried *** optimized extraction process was adopted to compare the contents of total flavonoids from different parts of *** Pursh.[Results]The best extraction process was extracting the powder of *** Pursh for 2.0 h with 20 times of 55%ethanol by reflux *** this condition,the contents of total flavonoids were 3.63%,8.90%,11.28%,and 4.36%from stems,leaves,flowers and whole grass of *** Pursh,respectively.[Conclusions]The process is reasonable,feasible and stable,and can effectively extract total flavonoids from *** *** contents of total flavonoids from different parts of *** Pursh were quite different,and the value was higher in the leaves and flowers,so the proportions of leaves and flowers should be paid attention to in the industrial processing of *** Pursh.