Morphological Tracing and Functional Identification of Monosynaptic Connections in the Brain:A Comprehensive Guide
作者机构：Affiliated Mental Health Center and Hangzhou Seventh People’s Hospital and School of Brain Science and Brain MedicineZhejiang University School of MedicineHangzhou310058China Liangzhu LaboratoryMOE Frontier Science Center for Brain Science and Brain-machine IntegrationState Key Laboratory of Brain-machine IntelligenceZhejiang UniversityHangzhou311121China NHC and CAMS Key Laboratory of Medical NeurobiologyZhejiang UniversityHangzhou310058China Department of Neurobiology and Department of Neurology of the Second Affiliated HospitalZhejiang University School of MedicineHangzhou310058China Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100005China
出 版 物：《Neuroscience Bulletin》 (神经科学通报（英文版）)
年 卷 期：2024年第40卷第9期
页 面：1364-1378页
核心收录：
基 金：National Key Research and Development Program of China(2022YFC2402703) Scientific and Technological Innovation 2030 Program of China,major projects(2021ZD0200408) National Natural Science Foundation of China(32171007 and 31800880) Non-profit Central Research Institute Fund of the Chinese Academy of Medical Sciences(2018PT31041) Science and Technology Planning Project of Guangdong Province(2018B030331001) Fundamental Research Funds for the Central Universities(K20210089)
主 题：Trans-monosynaptic Retrograde Anterograde Viral tool Neural circuit Optogenetics Electrophysiology
摘 要：Behavioral studies play a crucial role in unraveling the mechanisms underlying brain *** advances in optogenetics,neuronal typing and labeling,and circuit tracing have facilitated the dissection of the neural circuitry involved in various important *** identification of monosynaptic connections,both upstream and downstream of specific neurons,serves as the foundation for understanding complex neural circuits and studying behavioral ***,the practical implementation and mechanistic understanding of monosynaptic connection tracing techniques and functional identification remain challenging,particularly for inexperienced *** application of these methods and misinterpretation of results can impede experimental progress and lead to erroneous *** this paper,we present a comprehensive description of the principles,specific operational details,and key steps involved in tracing anterograde and retrograde monosynaptic *** outline the process of functionally identifying monosynaptic connections through the integration of optogenetics and electrophysiological techniques,providing practical guidance for researchers.
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