Wheat kinase TaSnRK2.4 forms a functional module with phosphatase TaPP2C01 and transcription factor TaABF2 to regulate drought response

作     者：Yanyang Zhang Xiaoyang Hou Tianjiao Li Ziyi Wang Jiaqi Zhang Chunlin Zhang Xianchang Liu Xinxin Shi Wanrong Duan Kai Xiao Yanyang Zhang;Xiaoyang Hou;Tianjiao Li;Ziyi Wang;Jiaqi Zhang;Chunlin Zhang;Xianchang Liu;Xinxin Shi;Wanrong Duan;Kai Xiao

作者机构：State Key Laboratory of North China Crop Improvement and RegulationBaoding 071001HebeiChina College of AgronomyHebei Agricultural UniversityBaoding 071001HebeiChina 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2024年第12卷第2期

页      面：384-400页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 

基　　金：supported by National Key Research and Development Program of China(2022YFD1200202) State Key Laboratory of North China Crop Improvement and Regulation(NCCIR2022ZZ-7) Graduate Student Innovation Ability Training Funding Project of Hebei Province(CXZZBS2023073) 

主　　题：Triticum aestivum SnRK2.4 kinase Gene expression Protein interaction Transgene analysis Transcriptional activation 

摘      要：SNF1-related protein kinase 2(SnRK2)family members are essential components of the plant abscisic acid(ABA)signaling pathway initiated by osmotic stress and triggering a drought stress *** study characterized the molecular properties of TaSnRK2.4 and its function in mediating adaptation to drought in Triticum *** of TaSnRK2.4 were upregulated upon drought and ABA signaling and associated with drought-and ABA-responsive cis-elements ABRE and DRE,and MYB and MYC binding sites in the promoter as indicated by reporter GUS protein staining and activity driven by truncations of the *** two-hybrid,BiFC,and Co-IP assays indicated that TaSnRK2.4 protein interacts with TaPP2C01 and an ABF transcription factor(TF)*** results suggested that TaSnRK2.4 forms a functional TaPP2C01-TaSnRK2.4-TaABF2 module with its upstream and downstream *** analysis revealed that TaSnRK2.4 and TaABF2 positively regulate drought tolerance whereas TaPP2C01 acts negatively by modulating stomatal movement,osmotic adjustment,reactive oxygen species(ROS)homeostasis,and root *** analysis,yeast one-hybrid,and transcriptional activation assays indicated that several osmotic stress-responsive genes,including TaSLAC1-4,TaP5CS3,TaSOD5,TaCAT1,and TaPIN4,are regulated by *** analysis verified their functions in positively regulating stomatal movement(TaSLAC1-4),proline accumulation(TaP5CS3),SOD activity(TaSOD5),CAT activity(TaCAT1),and root morphology(TaPIN4).There were high correlations between plant biomass and yield with module transcripts in a wheat variety panel cultivated under drought conditions in the *** findings provide insights into understanding plant drought response underlying the SnRK2 signaling pathway in common wheat.