Target-induced Trivalent G-quadruplex/hemin DNAzyme for Colorimetric Detection of Hg^(2+) Based on an Exonuclease III Assisted Catalytic Hairpin Assembly

作     者：Zhenghua LIU Zhonghai LI 

作者机构：College of Food Science and EngineeringCentral South University of Forestry and TechnologyChangsha410004China Technology Center of Changsha Customs District of People s Republic of ChinaChangsha410004China 

出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)

年 卷 期：2024年第13卷第1期

页      面：51-57页

学科分类：081704[工学-应用化学] 07[理学] 070304[理学-物理化学(含∶化学物理)] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 

基　　金：Supported by The Science and Technology Project of General Administration of Quality Supervision,Inspection and Quarantine (2015IK126) The Science and Technology Project of Changsha City of Hunan Province of China (KQ1602124) 

主　　题：G-quadruplex/hemin DNAzyme Multivalence Catalytic hairpin assembly Exonuclease III Signal amplification Colorimetric detection 

摘      要：Mercury ion(Hg^(2+)),a highly noxious of heavy metalion,has detrimental effects on the ecological environment and human ***,we have developed an exonuclease III(Exo III)assisted catalytic hairpin assembly formation of a trivalent G-quadruplex/hemin DNAzyme for colorimetric detection of Hg^(2+).A hairpin DNA(Hr)was designed with thymine-Hg^(2+)-thymine pairs that catalyzed by Exo III is prompted to happen upon binding Hg^(2+).A released DNA fragment triggers the catalytic assembly of other three hairpins(H1,H2,and H3)to form many trivalent G-quadruplex/hemin DNA enzymes for signal *** developed sensor shows a dynamic range from 2 pM to 2μM,with an impressively low detection limit of 0.32 pM for Hg^(2+)*** a sensor also has good selectivity toward Hg^(2+)detection in the presence of other common metal *** strategy shows the great potential for visual detection with portable type.