Toward predictable universal genetic circuit design

作     者：Yuanli Gao Baojun Wang Yuanli Gao;Baojun Wang

作者机构：College of Chemical and Biological EngineeringZhejiang UniversityHangzhouChina ZJU-Hangzhou Global Scientific and Technological Innovation CenterZhejiang UniversityHangzhouChina School of Biological SciencesUniversity of EdinburghEdinburghUK 

出 版 物：《Quantitative Biology》 (定量生物学（英文版）)

年 卷 期：2024年第12卷第2期

页      面：225-229页

核心收录：

学科分类：0710[理学-生物学] 08[工学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Fundamental Research Funds for the Central Universities,Grant/Award Number:226-2022-00214 National Key R&D Program of China,Grant/Award Number:2023YFF1204500 “Pioneer”and“Leading Goose”R&D Program of Zhejiang,Grant/Award Number:2024C03011 National Natural Science Foundation of China,Grant/AwardNumbers:32271475,32320103001 

主　　题：circuit predictability genetic circuit design host independent part characterization portability 

摘      要：In thepast 2decades,synthetic biologists have applied systematic engineering principles to genetic circuit design to devise biological systems with bespoke behaviors,such as Boolean logic gates,signal filters,oscillators,state machines,perceptrons,and genetic controllers[1,2].Following a bottom-up strategy,the genetic circuits are designed by assembling a set of well-characterized biological components,or genetic parts[3],and optimized through the iterative Design-Build-Test-Learn(DBTL)cycles.