Engineering a non-model yeast Rhodotorula mucilaginosa for terpenoids synthesis
作者机构：Laboratory of BiotechnologyDalian Institute of Chemical PhysicsCAS457 Zhongshan RoadDalian116023China MOE Key Laboratory of Bio-Intelligent ManufacturingSchool of BioengineeringDalian University of TechnologyDalian116024China University of Chinese Academy of Sciences19 Yuquan RoadBeijing100049China
出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)
年 卷 期：2024年第9卷第3期
页 面：569-576页
核心收录：
学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学]
基 金：National Key Research and Development Program of China(2021YFA0910600) National Natural Science Foundation of China(22308350,22238010) Liaoning Revitalization Talents Program(XLYC2002089) Science and Technology Bureau of Dalian City(2021RT04)
主 题：Rhodotorula mucilaginosa Synthetic biology α-Terpineol β-Ionone α-Farnesene
摘 要：Terpenoids have tremendous biological activities and are widely employed in food,healthcare and pharmaceutical *** synthetic biology to product terpenoids from microbial cell factories presents a promising alternative route compared to conventional methods such as chemical synthesis or *** red yeast Rhodotorula mucilaginosa has been widely studied due to its natural production capacity of carotenoid and lipids,indicating a strong endogenous isoprene pathway with readily available metabolic *** study constructed several engineered strains of *** with the aim of producing different ***α-terpineol was produced by expressing theα-terpineol synthase from Vitis *** titer ofα-terpineol was further enhanced to 0.39 mg/L by overexpressing the endogenous rate-limiting gene of the MVA *** ofα-farnesene synthase from Malus domestica,in combination with MVA pathway rate-limiting gene resulted in significant increase inα-farnesene production,reaching a titer of 822 mg/*** carotenoid degradation productβ-ionone was produced at a titer of 0.87 mg/L by expressing theβ-ionone synthase from Petunia *** study demonstrates the potential of *** as a platform host for the direct biosynthesis of various terpenoids and provides insights for further development of such platforms.
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