GmBES1-1 dampens the activity of GmNSP1/2 to mediate brassinosteroid inhibition of nodulation in soybean

作     者：Xu Chen Xiaotong Hu Haijiao Wang Jing Liu Yaqi Peng Chunmei He Miao He Xuelu Wang 

作者机构：Center of Integrative BiologyCollege of Life Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China State Key Laboratory of Crop Stress Adaptation and ImprovementSchool of Life SciencesHenan UniversityZhengzhou 450046China Academy for Advanced Interdisciplinary StudiesHenan UniversityZhengzhou 450046China Sanya Institute of Henan UniversitySanya 572025China 

出 版 物：《Plant Communications》 (植物通讯（英文）)

年 卷 期：2023年第4卷第6期

页      面：244-258页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：supported by grants from the Key Project of the Intergovernmental International Scientific and Technological Innovation Cooperation(2018YFE0112100 to X.W.) the National Natural Science Foundation of China(U21A20181,31870257 to X.W.) the Innovation Scientists and Technicians Troop Construction Projects of Henan Province(224000510001 to X.W.) the Special Fund for Scientific Research Development of Henan Academy of Sciences(220913002). 

主　　题：nodulation brassinosteroid crosstalk GmBES1-1 GmNSP1/2 

摘      要：Soybean(Glycine max)forms root nodules to house rhizobial bacteria for biological nitrogen fixation.The development of root nodules is intricately regulated by endogenous and exogenous cues.The phytohormones brassinosteroids(BRs)have been shown to negatively regulate nodulation in soybean,but the underlying genetic and molecular mechanisms remain largely unknown.Here,we performed transcriptomic analyses and revealed that BR signaling negatively regulates nodulation factor(NF)signaling.We found that BR signaling inhibits nodulation through its signaling component GmBES1-1 by dampening NF signaling and nodule formation.In addition,GmBES1-1 can directly interact with both GmNSP1 and GmNSP2 to inhibit their interaction and the DNA-binding activity ofGmNSP1.Furthermore,BR-induced nuclear accumulation of GmBES1-1 is essential for inhibiting nodulation.Taken together,our results demonstrate that regulation ofGmBES1-1 subcellular localization by BRs plays a key role in the legume–rhizobium symbiosis and plant development,indicating a crosstalk mechanism between phytohormone and symbiosis signaling pathways.