From Phenotypes to Molecules: Revolutionizing Gut Microbiota Identification Methods

作     者：WANG Xuan LV Chang-Long ZHAI Jing-Bo 王萱;吕昌龙;翟景波

作者机构：Medical CollegeInner Mongolia Minzu UniversityTongliao 028000Inner MongoliaChina Research Centre of Chemical BiologyYanbian UniversityYanji 133002JilinChina Key Laboratory of Zoonose Prevention and Control at Universities of Inner Mongolia Autonomous RegionTongliao 028000Inner MongoliaChina Institute of ImmunologyChina Medical UniversityShenyang 110000LiaoningChina Brucellosis Prevention and Treatment Engineering Research Center of Inner Mongolia Autonomous RegionTongliao 028000Inner MongoliaChina 

出 版 物：《中国生物化学与分子生物学报》 (Chinese Journal of Biochemistry and Molecular Biology)

年 卷 期：2024年第40卷第8期

页      面：1065-1077页

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学] 

基　　金：国家自然科学基金资助项目(No.82160312) 2023青年科技人才培养计划(创新团队)人兽共患病防控重点技术研究项目(No.NMGIRT2315) 内蒙古自治区重大科技项目(No.2019ZD006) 内蒙古自治区自然科学基金(No.2023MS08057) 内蒙古自治区高校人畜共患病防控重点实验室开放课题(No.MDK2023015,No.MDK2023017,No.MDK2023018)资助 

主　　题：gut microbiota 16S rRNA real-time fluorescent qPCR high-throughput sequencing mass spectrum 

摘      要：The gut microbiota is a complex ecosystem composed of many bacteria and their *** plays an irreplaceable role in human digestion,nutrient absorption,energy supply,fat metabolism,immune regulation,and many other *** the structure and function of the gut microbiota,as well as their key genes and metabolites,will enable the early diagnosis and auxiliary diagnosis of diseases,new treatment methods,better effects of drug treatments,and better guidance in the use of *** identification of gut microbiota plays an important role in clinical diagnosis and treatment,as well as in drug research and ***,it is necessary to conduct a comprehensive review of this rapidly evolving *** identification methods cannot comprehensively capture the diversity of gut ***,with the rapid development of molecular biology,the classification and identification methods for gut microbiota have evolved from the initial phenotypic and chemical identification to identification at the molecular *** review integrates the main methods of gut microbiota identification and evaluates their *** pay special attention to the research progress on molecular biological methods and focus on the application of high-throughput sequencing technology in the identification of gut *** revolutionary method for intestinal flora identification heralds a new chapter in our understanding of the microbial world.