Apoptosis of hepatoma cells SMMC-7721 induced by Ginkgo biloba seed polysaccharide
Apoptosis of hepatoma cells SMMC-7721 induced by Ginkgo biloba seed polysaccharide作者机构：Department of Biology Shandong Normal University Jinan 250014 Shandong Province China.
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2002年第8卷第5期
页 面：832-836页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：银杏籽多糖 肝癌 扫描电镜 SMMC-7721细胞 细胞凋亡
摘 要：AIM: To study the apoptosis of hepatoma cells SMMC-7721induced by polysaccharide isolated from Ginkgo biloba ***: Ginkgo biloba seed polysaccharide (GBSP) wasisolated by ethanol fractionation of Ginkgo biloba seed andpurified by Sephadex G-200 chromatography. The purity ofGBSP was verified by reaction with iodine-potassium iodideand ninhydrin and confirmed by UV spectrophotometer,cellulose acetate membrane electrophoresis and Sepharose4B gel filtration chromatography. The Scanning ElectronMicroscope (SEM) and Flow Cytometrv (FCM) were used toexamine the SMMC-7721 cells with and without GBSPtreatment at 500 mg/ml for 36 ***: GBSP product obtained was of high purity withthe average molecular weight of 1.86 × 105. Quantitativeanalysis of SMMC-7721 cells in vitro with FCM showed thatthe percentages of G2-M cells without and with GBSPtreatment were 17.01±1.28 % and 11.77±1.50% (P0.05),the debds ratio of the cells were 0.46±0.12 % and 0.06±0 .06 %(P0.01), and the apoptosis ratio of cells was 3.84±0 .55 %and 9.13±1.48 %(P0.01) respectively. Following GBSPtreatment, microvilli of SMMC-7721 cells appeared thinnerand the number of spherical cells increased markedly. Mostsignificantly, the apoptosis bodies were formed on andaround the spherical cells treated with ***: GBSP could potentially induce the apoptosisof SMMC-7721 cells.
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