Inula britannica ameliorates alcohol-induced liver injury by modulating SIRT1-AMPK/Nrf2/NF-κB signaling pathway

作     者：Zhennan Meng Mengyuan Li Xiaoli Wang Kuo Zhang Chunfu Wu Xiaoshu Zhang Zhennan Meng;Mengyuan Li;Xiaoli Wang;Kuo Zhang;Chunfu Wu;Xiaoshu Zhang

作者机构：Faculity of Functional Food and WineShenyang Pharmaceutical UniversityShenyang 110016China School of PharmacyShenyang Pharmaceutical UniversityShenyang 110016China 

出 版 物：《Chinese Herbal Medicines》 (中草药（英文版）)

年 卷 期：2024年第16卷第4期

页      面：667-678页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China (grant No. 81703389) Natural Science Foundation of Science and Technology Department of Liaoning Province (No. 2021-MS-215) Youth Development Support Plan of Shenyang Pharmaceutical University (No. ZQN2021010) 

主　　题：alcoholic liver injury inflammation Inula britannica L. sesquiterpenes SIRT1-AMPK/Nrf2 

摘      要：Objective: Inula britannica is a traditional Chinese medicinal and functional food with various effects such as anti-liver injury, hypoglycemia, antioxidants, and anti-tumor. The aim of this study was to investigate the protective effects and mechanisms of the ethanolic extract of I. britannica(EEIB) on alcohol-induced liver injury in ***: Fifty-six female C57BL/6 mice were randomly divided into seven groups: control group(Con),ethanol feeding model group(EtOH), Silibinin positive treatment group(EtOH + Silibinin 100 mg/kg),EEIB treatment group(EtOH + EEIB 100, 200, and 400 mg/kg), and EEIB control group(EEIB 400 mg/kg). The National Institute on Alcohol Abuse and Alcoholism(NIAAA) ethanol-feeding model was used to study the effects of EEIB on alcohol-induced lipid metabolism, inflammation, oxidative stress, and fibril formation in mice by histopathological evaluation, immunofluorescence staining, Western blotting analysis and molecular ***: EEIB reduced liver indices to different degrees to normal levels and improved liver morphology in mice. EEIB inhibited alcohol-induced liver injury by activating the sirtuin 1(SIRT1)-adenosine monophosphate-activated protein kinase(AMPK) signaling pathway in the liver of alcohol-fed mice, in which sesquiterpenes may be the potential active ingredients, and also down-regulated the expression of alpha-smooth muscle actin(a-SMA), collagen alpha(Collagen I), tumor necrosis factor-alpha(TNFa) and attenuated alcohol-induced liver injury. In addition, EEIB also activated the nuclear factor erythroid 2-related factor 2(Nrf2) signaling pathway, which alleviated alcohol-induced liver injury at the level of oxidative stress. Notably, the EEIB control group demonstrated that EEIB had no toxic effects in mice. EEIB reduced alcoholic liver injury in a dose-dependent manner. Its therapeutic efficacy was comparable to, if not better than, that of Silibinin when administered at a dose of 400 mg/***: EEIB showed sig