The genome-wide meiotic recombination landscape in ciliates and its implications for crossover regulation and genome evolution
作者机构：Key Laboratory of Aquatic Biodiversity and ConservationInstitute of HydrobiologyChinese Academy of SciencesWuhanHubei 430072China University of Chinese Academy of SciencesBeijing 100049China Institute of Human Genetics(IGH)CNRSUniversity of Montpellier34396 MontpellierFrance Key Laboratory of Breeding Biotechnology and Sustainable AquacultureChinese Academy of SciencesWuhanHubei 430072China Key Laboratory of Lake and Watershed Science for Water SecurityChinese Academy of SciencesNanjingJiangsu 210008China
出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)
年 卷 期：2024年第51卷第3期
页 面：302-312页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学]
基 金：supported by the Wuhan Branch,Supercomputing Center,Chinese Academy of Sciences,China supported by the National Aquatic Biological Resource Center(NABRC) supported by the Bureau of Frontier Sciences and Education,Chinese Academy of Sciences(ZDBS-LY-SM026) the National Natural Science Foundation of China(32370457,32122015,32130011,31900316,and 31900339) the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB0480000) PJA3 grant of ARC Foundation(ARCPJA2021060003830) Equipes 2022 grant of Foundation Recherche Medicale(EQU202203014651).
主 题：Meiotic recombination Hotspot Crossover interference Adaptive evolution
摘 要：Meiotic recombination is essential for sexual reproduction and its regulation has been extensively studied in many taxa.However,genome-wide recombination landscape has not been reported in ciliates and it remains unknown how it is affected by the unique features of ciliates:the synaptonemal complex(SC)-independent meiosis and the nuclear dimorphism.Here,we show the recombination landscape in the model ciliate Tetrahymena thermophila by analyzing single-nucleotide polymorphism datasets from 38 hybrid progeny.We detect 1021 crossover(CO)events(35.8 per meiosis),corresponding to an overall CO rate of 9.9 cM/Mb.However,gene conversion by non-crossover is rare(1.03 per meiosis)and not biased towards G or C alleles.Consistent with the reported roles of SC in CO interference,we find no obvious sign of CO interference.CO tends to occur within germ-soma common genomic regions and many of the 44 identified CO hotspots localize at the centromeric or subtelomeric regions.Gene ontology analyses show that CO hotspots are strongly associated with genes responding to environmental changes.We discuss these results with respect to how nuclear dimorphism has potentially driven the formation of the observed recombination landscape to facilitate environmental adaptation and the sharing of machinery among meiotic and somatic recombination.
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